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Product Info Summary
| SKU: | A06709 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-DNA-binding protein RFX2 RFX2 Antibody
SKU/Catalog Number
A06709
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DNA-binding protein RFX2 RFX2 Antibody (Catalog # A06709). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA-binding protein RFX2 RFX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06709)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RFX2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06709 is reactive to RFX2 in Human
Applications
A06709 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
79.987kDa
Background of RFX2
a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites.
Reith W., Mol. Cell. Biol. 14:1230-1244(1994).
Sjoeblom T., Science 314:268-274(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using RFX2 antibody A06709.
Protein Target Info & Infographic
Gene/Protein Information For RFX2 (Source: Uniprot.org, NCBI)
Gene Name
RFX2
Full Name
DNA-binding protein RFX2
Weight
79.987kDa
Superfamily
RFX family
Alternative Names
DNA binding protein RFX2; DNA-binding protein RFX2; FLJ14226; HLA class II regulatory factor RFX2; Regulatory factor X 2; regulatory factor X, 2 (influences HLA class II expression); trans-acting regulatory factor 2 RFX2 regulatory factor X2 DNA-binding protein RFX2|HLA class II regulatory factor RFX2|regulatory factor X 2|regulatory factor X, 2 (influences HLA class II expression)|trans-acting regulatory factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RFX2, check out the RFX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RFX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA-binding protein RFX2 RFX2 Antibody (A06709)
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No publications found for A06709
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-DNA-binding protein RFX2 RFX2 Antibody
Question
Here is the WB image, lot number and protocol we used for testis using anti-RFX2 antibody A06709. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-RFX2 antibody A06709 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The anti-RFX2 antibody (A06709) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-12
Question
Would anti-RFX2 antibody A06709 work on monkey IHC with brain?
V. Parker
Verified customer
Asked: 2019-05-30
Answer
Our lab technicians have not tested anti-RFX2 antibody A06709 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-RFX2 antibody A06709 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-30
Question
Do you have a BSA free version of anti-RFX2 antibody A06709 available?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RFX2 antibody A06709 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-07
Question
I have a question about product A06709, anti-RFX2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Parker
Verified customer
Asked: 2017-03-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06709 anti-RFX2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-29
Question
I was wanting to use your anti-RFX2 antibody for IHC for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
F. Parker
Verified customer
Asked: 2015-07-27
Answer
You can see on the product datasheet, A06709 anti-RFX2 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-27
Question
Is a blocking peptide available for product anti-RFX2 antibody (A06709)?
Z. Walker
Verified customer
Asked: 2015-06-11
Answer
We do provide the blocking peptide for product anti-RFX2 antibody (A06709). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-06-11
