Product Info Summary
SKU: | A06212 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-EIF3D Antibody
SKU/Catalog Number
A06212
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EIF3D Antibody (Catalog # A06212). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF3D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06212)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human EIF3D.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06212 is reactive to EIF3D in Human, Mouse
Applications
A06212 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
63.973kDa
Background of EIF3D
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. HAMAP-Rule MF_03003
Asano K., J. Biol. Chem. 272:27042-27052(1997).
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 3T3 cells, using EIF3D antibody A06212.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EIF3D antibody A06212.
Protein Target Info & Infographic
Gene/Protein Information For EIF3D (Source: Uniprot.org, NCBI)
Gene Name
EIF3D
Full Name
Eukaryotic translation initiation factor 3 subunit D
Weight
63.973kDa
Superfamily
eIF-3 subunit D family
Alternative Names
eIF3d; eIF3-p66; EIF3S7eIF3 p66; eIF-3-zeta; eIF3-zeta; Eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3, subunit D; MGC126526; MGC17258; translation initiation factor eIF3 p66 subunit EIF3D EIF3S7, eIF3-p66, eIF3-zeta eukaryotic translation initiation factor 3 subunit D eukaryotic translation initiation factor 3 subunit D|eIF3 p66|eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa|translation initiation factor eIF3 p66 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF3D, check out the EIF3D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF3D Antibody (A06212)
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No publications found for A06212
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