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Product Info Summary
| SKU: | A13058 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Rim3 RIMS3 Antibody
SKU/Catalog Number
A13058
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Rim3 RIMS3 Antibody (Catalog # A13058). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Rim3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Rim3 RIMS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13058)
Host
Rabbit
Contents
Rim3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rim3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Rim3. The immunogen is located within amino acids 60 - 110 of Rim3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to other Rim proteins.
Reactive Species
A13058 is reactive to RIMS3 in Human, Mouse
Applications
A13058 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.796kDa
Background of RIMS3
Rab3-interacting molecules (RIMs) are synaptic proteins are synaptic proteins necessary for neural transmission and plasticity. While both Rim1 and Rim 2 are thought to be effector proteins for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner, less is known of Rim3. Expression of Rim3 in PC12 cells induced a significant increase in calcium-triggered exocytosis, with no appreciable change in the baseline release, suggesting that it plays a role in the regulation of exocytosis. Rim3 protein localizes primarily to neuronal dendrites and the postsynaptic densities, as opposed to Rim1 which is found in presynapse locations, indicating that Rim3 may contribute to synapse transmission and plasticity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Rim3 antibody can be used for detection of Rim3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Rim3 in human brain tissue lysate with Rim3 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
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Immunohistochemistry of Rim3 in human brain tissue with Rim3 antibody at 2.5 μg/mL.
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Immunofluorescence of rim3 in human brain tissue with rim3 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RIMS3 (Source: Uniprot.org, NCBI)
Gene Name
RIMS3
Full Name
Regulating synaptic membrane exocytosis protein 3
Weight
32.796kDa
Alternative Names
KIAA0137; KIAA0237; Nim3; NIM3Rab-3 interacting molecule 3; Rab-3-interacting molecule 3; regulating synaptic membrane exocytosis 3; regulating synaptic membrane exocytosis protein 3; RIM 3; RIM3 gamma; RIM3 RIMS3 NIM3, RIM 3, RIM3 regulating synaptic membrane exocytosis 3 regulating synaptic membrane exocytosis protein 3|RIM3 gamma|Rab-3 interacting molecule 3|rab-3-interacting molecule 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIMS3, check out the RIMS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIMS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rim3 RIMS3 Antibody (A13058)
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No publications found for A13058
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