Anti-RING1B RNF2 Antibody

Product Name

Anti-RING1B RNF2 Antibody

View all RNF2 Antibodies

SKU/Catalog Number

A01209-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-RING1B RNF2 Antibody (Catalog # A01209-1). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Chinese Hamster.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-RING1B RNF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01209-1)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01209-1 is reactive to RNF2 in Human, Mouse, Chinese hamster

Applications

A01209-1 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

37.655kDa

Background of RNF2

RING1B (also known as BAP1 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity. Anti-RINGB1 Antibody is useful for researchers interested in epigenetics, ubiquitin, and transcription factor research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
IF Microscopy: 1:300
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Immunofluorescence Microscopy of Goat anti-RING1B antibody.Tissue:human HeLa cells.Fixation:methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°

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Figure 2. Western blot analysis of RNF2 using anti-RNF2 antibody (A01209-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF2 antigen affinity purified polyclonal antibody (Catalog # A01209-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for RNF2.

Gene/Protein Information For RNF2 (Source: Uniprot.org, NCBI)

Alternative Names

BAP-1; BAP1RING finger protein 1B; DING; DINGRING finger protein BAP-1; EC 6.3.2; HIPI3; HIPI3RING1BE3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; Protein DinG; ring finger protein 2HIP2-interacting protein 3; RING1B; RING2; RING2EC 6.3.2.-; RNF2 RNF2 BAP-1, BAP1, DING, HIPI3, RING1B, RING2 ring finger protein 2 E3 ubiquitin-protein ligase RING2|HIP2-interacting protein 3|RING finger protein 1B|RING finger protein BAP-1|RING-type E3 ubiquitin transferase RING2|huntingtin-interacting protein 2-interacting protein 3|protein DinG

For more info on RNF2, check out the RNF2 Infographic

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In this infographic, you will see the following information for RNF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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