Anti-RPL19 Antibody Picoband™

Product Name

Anti-RPL19 Antibody Picoband™

View all RPL19 Antibodies

SKU/Catalog Number

PB10092

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RPL19 Antibody Picoband™ catalog # PB10092. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPL19 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10092)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RPL19, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10092 is reactive to RPL19 in Human, Mouse, Rat

Applications

PB10092 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

23.466kDa

Background of RPL19

60S ribosomal protein L19 is a protein that in humans is encoded by the RPL19 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L19E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RPL19 using anti-RPL19 antibody (PB10092).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: HEPA whole cell lysates,
Lane 3: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL19 antigen affinity purified polyclonal antibody (Catalog # PB10092) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL19 at approximately 28 kDa. The expected band size for RPL19 is at 23 kDa.

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Figure 2. IF analysis of RPL19 using anti-RPL19 antibody (PB10092).
RPL19 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RPL19 Antibody (PB10092) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-RPL19 antibody (PB10092).
Overlay histogram showing U20S cells stained with PB10092 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL19 Antibody (PB10092,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 4. Flow Cytometry analysis of A431 cells using anti-RPL19 antibody (PB10092).
Overlay histogram showing A431 cells stained with PB10092 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL19 Antibody (PB10092,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RPL19 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp779D216; FLJ27452; MGC71997,60S ribosomal protein L19; ribosomal protein L19; ribosomal protein L19, cytosolic, N-terminus truncated RPL19 L19 ribosomal protein L19 60S ribosomal protein L19|large ribosomal subunit protein eL19

For more info on RPL19, check out the RPL19 Infographic

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In this infographic, you will see the following information for RPL19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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