Anti-RPLP2/Ribosomal Protein Lp2 Antibody

RPLP2 antibody

Boster Bio Anti-RPLP2/Ribosomal Protein Lp2 Antibody (Catalog# A05244-1). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05244-1
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, IF (paraffin)

Product Name

Anti-RPLP2/Ribosomal Protein Lp2 Antibody

View all RPLP2 Antibodies

SKU/Catalog Number

A05244-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-RPLP2/Ribosomal Protein Lp2 Antibody (Catalog# A05244-1). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPLP2/Ribosomal Protein Lp2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05244-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human RPLP2. Immunogen sequence location: 21-70

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05244-1 is reactive to RPLP2 in Human, Mouse, Rat

Applications

A05244-1 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

11.665kDa

Background of RPLP2

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For RPLP2 (Source: Uniprot.org, NCBI)

Gene Name

RPLP2

Full Name

60S acidic ribosomal protein P2

Weight

11.665kDa

Superfamily

eukaryotic ribosomal protein P1/P2 family

Alternative Names

acidic ribosomal phosphoprotein P2; MGC71408,60S acidic ribosomal protein P2; P2; Renal carcinoma antigen NY-REN-44; ribosomal protein, large, P2; RPP2D11S2243E RPLP2 D11S2243E, LP2, P2, RPP2 ribosomal protein lateral stalk subunit P2 60S acidic ribosomal protein P2|acidic ribosomal phosphoprotein P2|large ribosomal subunit protein P2|renal carcinoma antigen NY-REN-44|ribosomal protein, large, P2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPLP2, check out the RPLP2 Infographic

RPLP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPLP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-RPLP2/Ribosomal Protein Lp2 Antibody

Question

Can you help my question with product A05244-1, anti-RPLP2/Ribosomal Protein Lp2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05244-1 anti-RPLP2/Ribosomal Protein Lp2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-22

Question

Do you have a BSA free version of anti-RPLP2/Ribosomal Protein Lp2 antibody A05244-1 available?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RPLP2/Ribosomal Protein Lp2 antibody A05244-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-05

Question

Is a blocking peptide available for product anti-RPLP2/Ribosomal Protein Lp2 antibody (A05244-1)?

C. Moore

Verified customer

Asked: 2019-09-23

Answer

We do provide the blocking peptide for product anti-RPLP2/Ribosomal Protein Lp2 antibody (A05244-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-23

Question

Will anti-RPLP2/Ribosomal Protein Lp2 antibody A05244-1 work for IHC with liver?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

According to the expression profile of liver, RPLP2 is highly expressed in liver. So, it is likely that anti-RPLP2/Ribosomal Protein Lp2 antibody A05244-1 will work for IHC with liver.

Boster Scientific Support

Answered: 2019-06-03

Question

We are currently using anti-RPLP2/Ribosomal Protein Lp2 antibody A05244-1 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

The anti-RPLP2/Ribosomal Protein Lp2 antibody (A05244-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-15

Question

I see that the anti-RPLP2/Ribosomal Protein Lp2 antibody A05244-1 works with IHC, what is the protocol used to produce the result images on the product page?

J. Zhao

Verified customer

Asked: 2014-03-13

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-03-13

Order DetailsPrice
A05244-1

100 μL

$335

Free Secondary Antibody

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A05244-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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