Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband™ Antibody

Product Name

Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband™ Antibody

View all RSK2 Antibodies

SKU/Catalog Number

A02215-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband™ Antibody catalog # A02215-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02215-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Rsk 2/MAPKAP Kinase 1b/RPS6KA3 recombinant protein (Position: D393-K733).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02215-2 is reactive to RPS6KA3 in Human, Mouse, Rat

Applications

A02215-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

83.736kDa

Background of RSK2

Ribosomal protein S6 kinase, 90kDa, polypeptide 3, also known as RPS6KA3, is an enzyme that in humans is encoded by the RPS6KA3 gene. It is mapped to Xp22.12. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: human A431 whole cell lysates,
Lane 8: human HEK293 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (Catalog # A02215-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA3 at approximately 90KD. The expected band size for RPS6KA3 is at 90KD.

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Figure 2. Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (Catalog # A02215-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA3 at approximately 90KD. The expected band size for RPS6KA3 is at 90KD.

Gene/Protein Information For RPS6KA3 (Source: Uniprot.org, NCBI)

Alternative Names

90 kDa ribosomal protein S6 kinase 3; CLS; Coffin-Lowry syndrome; EC 2.7.11; EC 2.7.11.1; HU-3; Insulin-stimulated protein kinase 1; ISPK1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK1B; MAPKAPK-1b; mental retardation, X-linked 19; MRX19; p90-RSK2; p90RSK3; pp90RSK2; ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kD, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 2; RPS6KA3; RSK; RSK2; RSK-2; RSK2p90-RSK 3; S6K-alpha3; S6K-alpha-3 RPS6KA3 CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2 ribosomal protein S6 kinase A3 ribosomal protein S6 kinase alpha-3|MAP kinase-activated protein kinase 1b|MAPK-activated protein kinase 1b|MAPKAP kinase 1b|MAPKAPK-1b|RSK-2|S6K-alpha-3|epididymis secretory sperm binding protein|insulin-stimulated protein kinase 1|p90-RSK 3|ribosomal S6 kinase 2|ribosomal protein S6 kinase, 90kDa, polypeptide 3

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