Product Info Summary
SKU: | M02215 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Rsk-2 RPS6KA3 Monoclonal Antibody
SKU/Catalog Number
M02215
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rsk-2 RPS6KA3 Monoclonal Antibody catalog # M02215. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rsk-2 RPS6KA3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02215)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
40000000000
Isotype
IgG
Immunogen
Purified recombinant fragment of human Rsk-2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02215 is reactive to RPS6KA3 in Human
Applications
M02215 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
83.736kDa
Background of RSK2
LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. ELISA validation of RPS6KA3 using Anti-Rsk-2 RPS6KA3 Monoclonal Antibody (M02215).
ELISA analysis of Rsk-2 antibody.Click image to see more details
Figure 3. Flow Cytometry validation of RPS6KA3 using Anti-Rsk-2 RPS6KA3 Monoclonal Antibody (M02215).
Flow cytometric (FCM) analysis of HepG2 cells using Rsk-2 Monoclonal Antibody (green) and negative control (red).Click image to see more details
Figure 2. Immunofluorescent staining data of RPS6KA3 using Anti-Rsk-2 RPS6KA3 Monoclonal Antibody (M02215).
Immunofluorescence (IF) analysis of HepG2 cells using Rsk-2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 1. Western blotting validation for Anti-Rsk-2 RPS6KA3 Monoclonal Antibody M02215
Western Blot (WB) analysis using Rsk-2 Monoclonal Antibody against HeLa (1)Protein Target Info & Infographic
Gene/Protein Information For RPS6KA3 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA3
Full Name
Ribosomal protein S6 kinase alpha-3
Weight
83.736kDa
Superfamily
protein kinase superfamily
Alternative Names
90 kDa ribosomal protein S6 kinase 3; CLS; Coffin-Lowry syndrome; EC 2.7.11; EC 2.7.11.1; HU-3; Insulin-stimulated protein kinase 1; ISPK1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK1B; MAPKAPK-1b; mental retardation, X-linked 19; MRX19; p90-RSK2; p90RSK3; pp90RSK2; ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kD, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 2; RPS6KA3; RSK; RSK2; RSK-2; RSK2p90-RSK 3; S6K-alpha3; S6K-alpha-3 RPS6KA3 CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2 ribosomal protein S6 kinase A3 ribosomal protein S6 kinase alpha-3|MAP kinase-activated protein kinase 1b|MAPK-activated protein kinase 1b|MAPKAP kinase 1b|MAPKAPK-1b|RSK-2|S6K-alpha-3|epididymis secretory sperm binding protein|insulin-stimulated protein kinase 1|p90-RSK 3|ribosomal S6 kinase 2|ribosomal protein S6 kinase, 90kDa, polypeptide 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA3, check out the RPS6KA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rsk-2 RPS6KA3 Monoclonal Antibody (M02215)
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No publications found for M02215
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