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Product Info Summary
| SKU: | A06301-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-S100A14 Antibody Picoband™
View all S100 calcium binding protein A14 Antibodies
SKU/Catalog Number
A06301-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-S100A14 Antibody Picoband™ catalog # A06301-1. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-S100A14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06301-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human S100A14, which shares 93.8% amino acid (aa) sequence identity with mouse S100A14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06301-1 is reactive to S100A14 in Human
Applications
A06301-1 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
18 kDa
Calculated molecular weight
11.662kDa
Background of S100 calcium binding protein A14
S100 calcium binding protein A14 (S100A14) is a protein that in humans is encoded by the S100A14 gene. This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. IHC analysis of S100A14 using anti-S100A14 antibody (A06301-1).
S100A14 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A14 Antibody (A06301-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of S100A14 using anti-S100A14 antibody (A06301-1).
S100A14 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A14 Antibody (A06301-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For S100A14 (Source: Uniprot.org, NCBI)
Gene Name
S100A14
Full Name
Protein S100-A14
Weight
11.662kDa
Superfamily
S-100 family
Alternative Names
protein S100-A14; S100 calcium binding protein A14; S100 calcium-binding protein A14; S100A15BCMP84breast cancer membrane protein 84; S114 S100A14 BCMP84, S100A15 S100 calcium binding protein A14 protein S100-A14|S114|breast cancer membrane protein 84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100A14, check out the S100A14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100A14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S100A14 Antibody Picoband™ (A06301-1)
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No publications found for A06301-1
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Customer Reviews
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