Anti-SAPS3/PPP6R3 Antibody Picoband™

Product Name

Anti-SAPS3/PPP6R3 Antibody Picoband™

View all SAPS3 Antibodies

SKU/Catalog Number

A10833-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SAPS3/PPP6R3 Antibody Picoband™ catalog # A10833-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SAPS3/PPP6R3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10833-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human SAPS3/PPP6R3 recombinant protein (Position: R279-R848).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10833-1 is reactive to Ppp6r3 in Human, Monkey, Mouse, Rat

Applications

A10833-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

144 kDa

Calculated molecular weight

94.653kDa

Background of SAPS3

Protein phosphatase regulatory subunits, such as SAPS3, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS3 is a regulatory subunit for the protein phosphatase-6 catalytic subunit.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SAPS3/PPP6R3 using anti-SAPS3/PPP6R3 antibody (A10833-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAPS3/PPP6R3 antigen affinity purified polyclonal antibody (Catalog # A10833-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAPS3/PPP6R3 at approximately 144 kDa. The expected band size for SAPS3/PPP6R3 is at 95 kDa.

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Figure 2. IF analysis of PPP6R3 using anti-PPP6R3 antibody (A10833-1) and anti-Beta Tubulin antibody (M01857-3).
PPP6R3 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP6R3 Antibody (A10833-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of Hela cells using anti-SAPS3/PPP6R3 antibody (A10833-1).
Overlay histogram showing Hela cells stained with A10833-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAPS3/PPP6R3 Antibody (A10833-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Ppp6r3 (Source: Uniprot.org, NCBI)

Alternative Names

C11orf23SAPLa; chromosome 11 open reading frame 23; DKFZp781E17107; DKFZp781E2374; DKFZp781O2362; FLJ11058; FLJ43065; KIAA1558MGC125711; PP6R3MGC125712; protein phosphatase 6, regulatory subunit 3; SAPLSAP190; SAPS domain family member 3; SAPS3SAPS domain family, member 3; serine/threonine-protein phosphatase 6 regulatory subunit 3; Sporulation-induced transcript 4-associated protein SAPL Ppp6r3|4930528G08Rik, 9130026N02Rik, D19Bwg1430e, D19Ertd703, D19Ertd703e, Pp, Pp6r3, Ppcs3, Ppt, Pptcs3, S, Sapl, Saps3, mKIAA1558|protein phosphatase 6, regulatory subunit 3|serine/threonine-protein phosphatase 6 regulatory subunit 3|SAPS domain family, member 3|pluripotent cell-specific 3|sporulation-induced transcript 4-associated protein

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In this infographic, you will see the following information for Ppp6r3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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