Anti-SCAMP1 Antibody Picoband™

Product Name

Anti-SCAMP1 Antibody Picoband™

View all SCAMP1 Antibodies

SKU/Catalog Number

A08692-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SCAMP1 Antibody Picoband™ catalog # A08692-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SCAMP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08692-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SCAMP1 recombinant protein (Position: M1-N257).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08692-1 is reactive to SCAMP1 in Human, Mouse, Rat

Applications

A08692-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

37.92kDa

Background of SCAMP1

Secretory carrier-associated membrane protein 1 is a protein that in humans is encoded by the SCAMP1 gene. This gene product belongs to the SCAMP family of proteins, which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that these protein family members may function at the same site during vesicular transport rather than in separate pathways. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SCAMP1 using anti-SCAMP1 antibody (A08692-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human T98G whole cell lysates,
Lane 4: rat pancreas tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse pancreas tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAMP1 antigen affinity purified polyclonal antibody (Catalog # A08692-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCAMP1 at approximately 35 kDa. The expected band size for SCAMP1 is at 35 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of U87 cells using anti-SCAMP1 antibody (A08692-1).
Overlay histogram showing U87 cells stained with A08692-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCAMP1 Antibody (A08692-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SCAMP1 (Source: Uniprot.org, NCBI)

Alternative Names

secretory carrier membrane protein 1SCAMP37SCAMP; secretory carrier-associated membrane protein 1 SCAMP1 SCAMP, SCAMP37 secretory carrier membrane protein 1 secretory carrier-associated membrane protein 1

For more info on SCAMP1, check out the SCAMP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SCAMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the SCAMP1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis