Anti-SCNN1D Antibody

SCNN1D antibody

Boster Bio Anti-SCNN1D Antibody (Catalog # A07883-1). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A07883-1
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-SCNN1D Antibody

View all SCNN1D Antibodies

SKU/Catalog Number

A07883-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-SCNN1D Antibody (Catalog # A07883-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SCNN1D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07883-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SCNN1D.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07883-1 is reactive to SCNN1D in Human

Applications

A07883-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

87.85kDa

Background of SCNN1D

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

Waldmann R., J. Biol. Chem. 270:27411-27414(1995).
Ota T., Nat. Genet. 36:40-45(2004).
Gregory S.G., Nature 441:315-321(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For SCNN1D (Source: Uniprot.org, NCBI)

Gene Name

SCNN1D

Full Name

Amiloride-sensitive sodium channel subunit delta

Weight

87.85kDa

Superfamily

amiloride-sensitive sodium channel (TC 1.A.6) family

Alternative Names

Delta-ENaC; Delta-NaCH; DNACH; ENaCd; MGC149710; MGC149711; SCNED; sodium channel, nonvoltage-gated 1, delta; voltage-gated, type I, delta polypeptide SCNN1D ENaCd, ENaCdelta, SCNED, dNaCh sodium channel epithelial 1 subunit delta amiloride-sensitive sodium channel subunit delta|delta-ENaC|delta-NaCH|epithelial Na(+) channel subunit delta|nonvoltage-gated sodium channel 1 subunit delta|sodium channel epithelial 1 delta subunit|sodium channel, non-voltage-gated 1, delta subunit|sodium channel, nonvoltage-gated 1, delta|sodium channel, voltage-gated, type I, delta polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SCNN1D, check out the SCNN1D Infographic

SCNN1D infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SCNN1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-SCNN1D Antibody

Question

I see that the anti-SCNN1D antibody A07883-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-06

Question

Does anti-SCNN1D antibody A07883-1 work for WB with hippocampus testis?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

According to the expression profile of hippocampus testis, SCNN1D is highly expressed in hippocampus testis. So, it is likely that anti-SCNN1D antibody A07883-1 will work for WB with hippocampus testis.

Boster Scientific Support

Answered: 2020-01-20

Question

Will anti-SCNN1D antibody A07883-1 work on pig IF with brain?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

Our lab technicians have not tested anti-SCNN1D antibody A07883-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SCNN1D antibody A07883-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-31

Question

Would A07883-1 anti-SCNN1D antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

It shows on the product datasheet, A07883-1 anti-SCNN1D antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-01

Question

Please see the WB image, lot number and protocol we used for hippocampus testis using anti-SCNN1D antibody A07883-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-08

Question

I was wanting to use your anti-SCNN1D antibody for WB for human hippocampus testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus testis identification?

Verified Customer

Verified customer

Asked: 2018-11-08

Answer

As indicated on the product datasheet, A07883-1 anti-SCNN1D antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-08

Question

We are currently using anti-SCNN1D antibody A07883-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?

K. Anderson

Verified customer

Asked: 2013-12-10

Answer

The anti-SCNN1D antibody (A07883-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-12-10

Order DetailsPrice
A07883-1-100uL

100uL

$360

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A07883-1
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