Anti-SCTR Antibody Picoband™

Product Name

Anti-SCTR Antibody Picoband™

View all Secretin R Antibodies

SKU/Catalog Number

PB10096

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SCTR Antibody Picoband™ catalog # PB10096. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SCTR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10096)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SCTR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10096 is reactive to SCTR in Human, Rat

Applications

PB10096 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

59 kDa

Calculated molecular weight

50.207kDa

Background of Secretin R

Human secretin receptor (gene name SCTR) is a G protein-coupled receptor and belongs to the glucagon-VIP-secretin receptor family. It binds secretin which is the most potent regulator of pancreatic bicarbonate, electrolyte and volume secretion. Secretin and its receptor are suggested to be involved in pancreatic cancer and autism. The SCTR gene is mapped to chromosome 2q14.1 by fluorescence in situ hybridization.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SCTR using anti-SCTR antibody (PB10096).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: SKOV3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCTR antigen affinity purified polyclonal antibody (Catalog # PB10096) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCTR at approximately 59 kDa. The expected band size for SCTR is at 50 kDa.

Gene/Protein Information For SCTR (Source: Uniprot.org, NCBI)

Alternative Names

pancreatic secretin receptor; SCTR; SCT-R; Secretin R; secretin receptor; SecretinR; SR SCTR SR secretin receptor secretin receptor|pancreatic secretin receptor

For more info on SCTR, check out the SCTR Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SCTR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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