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Product Info Summary
| SKU: | A01261 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SDHD Antibody
SKU/Catalog Number
A01261
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SDHD Antibody (Catalog # A01261). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SDHD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SDHD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01261)
Host
Rabbit
Contents
SDHD Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.
Reactive Species
A01261 is reactive to SDHD in Human, Mouse, Rat
Applications
A01261 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
17.043kDa
Background of SDHD
The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of SDHD in EL4 cells with SDHD antibody at 2.5 μg/mL.
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Immunofluorescence of SDHD in EL4 cells with SDHD antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SDHD (Source: Uniprot.org, NCBI)
Gene Name
SDHD
Full Name
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Weight
17.043kDa
Superfamily
CybS family
Alternative Names
CBT1; CII-4; cybS; PGL; PGL1; QPs3; SDH4; succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial; Succinate dehydrogenase complex subunit D; succinate dehydrogenase complex, subunit D, integral membrane protein; succinate dehydrogenase ubiquinone cytochrome B small subunit; Succinate-ubiquinone oxidoreductase cytochrome b small subunit; Succinate-ubiquinone reductase membrane anchor subunit SDHD CBT1, CII-4, CWS3, MC2DN3, PGL, PGL1, QPs3, SDH4, cybS succinate dehydrogenase complex subunit D succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial|succinate dehydrogenase complex subunit D integral membrane protein|succinate-ubiquinone oxidoreductase cytochrome b small subunit|succinate-ubiquinone reductase membrane anchor subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDHD, check out the SDHD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDHD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDHD Antibody (A01261)
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No publications found for A01261
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Customer Reviews
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