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Product Info Summary
| SKU: | A07848 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SENP8/Nedp1 Antibody
View all NEDP1/SENP8 Antibodies
SKU/Catalog Number
A07848
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SENP8/Nedp1 Antibody (Catalog # A07848). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SENP8/Nedp1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07848)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human SENP8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07848 is reactive to Senp8 in Human, Mouse, Rat
Applications
A07848 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
25.057kDa
Background of NEDP1/SENP8
Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
Tudeviin Gan-Erdene, J. Biol. Chem., Aug 2003; 278: 28892 - 28900.
Debaditya Mukhopadhyay, J. Cell Biol., Sep 2006; 174: 939 - 949.
Thomas Colby, Plant Physiology, Sep 2006; 142: 318 - 332.
Joris Hemelaar, Mol. Cell. Biol., Jan 2004; 24: 84 - 95.<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HuvEc cells, using SENP8 antibody A07848.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SENP8 antibody A07848.
Protein Target Info & Infographic
Gene/Protein Information For Senp8 (Source: Uniprot.org, NCBI)
Gene Name
Senp8
Full Name
Sentrin-specific protease 8
Weight
25.057kDa
Superfamily
peptidase C48 family
Alternative Names
DEN1; DEN1deneddylase-1; deneddylase 1; Deneddylase-1; EC 3.4.22.-; HsT17512; NEDD8-specific protease 1; NEDP1; NEDP1NEDD8 specific-protease cysteine 2; Protease, cysteine 2; protease, cysteine, 2 (NEDD8 specific); PRSC2; SENP8; Sentrin/SUMO-specific protease SENP8; sentrin-specific protease 8; SUMO sentrin specific protease family member 8; SUMO/sentrin specific peptidase family member 8; SUMO/sentrin specific protease family member 8 Senp8|9130010J17Rik, AU020827, Den, Den1, Ned, Nedp1, Pr, Prsc2|SUMO/sentrin specific peptidase 8|sentrin-specific protease 8|NEDD8-specific protease 1|SUMO/sentrin specific protease 8|SUMO/sentrin specific protease family member 8|deneddylase-1|protease, cysteine, 2 (NEDD8 specific)|sentrin/SUMO-specific protease SENP8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Senp8, check out the Senp8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Senp8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SENP8/Nedp1 Antibody (A07848)
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No publications found for A07848
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Recommended Resources
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SENP8/Nedp1 Antibody
Question
Will A07848 anti-SENP8/Nedp1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, A07848 anti-SENP8/Nedp1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-SENP8/Nedp1 antibody A07848. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
I was wanting to use your anti-SENP8/Nedp1 antibody for WB for rat kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat kidney identification?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
It shows on the product datasheet, A07848 anti-SENP8/Nedp1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-14
Question
My question regarding product A07848, anti-SENP8/Nedp1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07848 anti-SENP8/Nedp1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-26
Question
Will anti-SENP8/Nedp1 antibody A07848 work on primate IHC with brain?
L. Krishna
Verified customer
Asked: 2016-05-23
Answer
Our lab technicians have not validated anti-SENP8/Nedp1 antibody A07848 on primate. You can run a BLAST between primate and the immunogen sequence of anti-SENP8/Nedp1 antibody A07848 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-23
Question
We are currently using anti-SENP8/Nedp1 antibody A07848 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
P. Carter
Verified customer
Asked: 2015-12-23
Answer
The anti-SENP8/Nedp1 antibody (A07848) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-23
