Anti-Phospho-SH3BP2 S427 Antibody

SH3BP2 antibody

Boster Bio Anti-Phospho-SH3BP2 S427 Antibody (Catalog # P04008). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P04008
Size: 100ug
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Phospho-SH3BP2 S427 Antibody

View all SH3BP2 Antibodies

SKU/Catalog Number

P04008

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-SH3BP2 S427 Antibody (Catalog # P04008). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Phospho-SH3BP2 S427 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04008)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P04008 is reactive to SH3BP2 in Human, Mouse, Rat

Applications

P04008 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

62.244kDa

Background of SH3BP2

SH3BP2 Src Homology 3 Binding Protein 2 is also known as 3BP-2 and SH3 Binding Protein 2. The Src homology 3 (SH3) region is a small protein domain presented in a very large group of proteins, including cytoskeletal elements and signaling proteins. SH3 domains are believed to serve as modules that mediate protein-protein associations and, along with Src homology 2 (SH2) domains, regulate cytoplasmic signaling. SH3BP2 is composed of an N terminal pleckstrin homology (PH) domain, a ten aa SH3 binding domain, three modular peptide recognition domains, and a C terminal SH2 domain. SH3BP2 function relates to signal transduction and regulation. SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Phosphorylation of SH3BP2 occurs on S427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase). Defects in SH3BP2 are the cause of cherubism (CRBM), an autosomal dominant inherited syndrome. It is characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For SH3BP2 (Source: Uniprot.org, NCBI)

Gene Name

SH3BP2

Full Name

SH3 domain-binding protein 2

Weight

62.244kDa

Alternative Names

Abl-SH3 binding protein 2; Cherubism; CRBM; CRPM; FLJ42079; FLJ54978,3BP-2,3BP2; RES4-23; SH3 domain-binding protein 2; SH3-domain binding protein 2; TNFAIP3 interacting protein 2 SH3BP2 3BP-2, 3BP2, CRBM, CRPM, RES4-23 SH3 domain binding protein 2 SH3 domain-binding protein 2|Abl-SH3 binding protein 2|TNFAIP3 interacting protein 2|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SH3BP2, check out the SH3BP2 Infographic

SH3BP2 infographic

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In this infographic, you will see the following information for SH3BP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Phospho-SH3BP2 S427 Antibody

Question

We are currently using anti-Phospho-SH3BP2 S427 antibody P04008 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-Phospho-SH3BP2 S427 antibody (P04008) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Question

Is a blocking peptide available for product anti-Phospho-SH3BP2 S427 antibody (P04008)?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

We do provide the blocking peptide for product anti-Phospho-SH3BP2 S427 antibody (P04008). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-12

Question

Here is the WB image, lot number and protocol we used for cervix using anti-Phospho-SH3BP2 S427 antibody P04008. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-20

Order DetailsPrice
P04008

100 μg

$599

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