Product Info Summary
SKU: | P04008 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-SH3BP2 S427 Antibody
SKU/Catalog Number
P04008
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Phospho-SH3BP2 S427 Antibody (Catalog # P04008). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Phospho-SH3BP2 S427 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04008)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P04008 is reactive to SH3BP2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
62244 MW
Background of SH3BP2
SH3BP2 Src Homology 3 Binding Protein 2 is also known as 3BP-2 and SH3 Binding Protein 2. The Src homology 3 (SH3) region is a small protein domain presented in a very large group of proteins, including cytoskeletal elements and signaling proteins. SH3 domains are believed to serve as modules that mediate protein-protein associations and, along with Src homology 2 (SH2) domains, regulate cytoplasmic signaling. SH3BP2 is composed of an N terminal pleckstrin homology (PH) domain, a ten aa SH3 binding domain, three modular peptide recognition domains, and a C terminal SH2 domain. SH3BP2 function relates to signal transduction and regulation. SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Phosphorylation of SH3BP2 occurs on S427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase). Defects in SH3BP2 are the cause of cherubism (CRBM), an autosomal dominant inherited syndrome. It is characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04008 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IP: 1:100
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SH3BP2 expression in M059 whole cell lysates(left) and PC-3 whole cell lysates (right). SH3BP2 was detected using rabbit anti-SH3BP2 pS427 Antigen Affinity purified polyclonal antibody (Catalog # P04008) at 1:500. Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). Personal communication Steven Pelech, Kinexus Inc.
Protein Target Info & Infographic
Gene/Protein Information For SH3BP2 (Source: Uniprot.org, NCBI)
Gene Name
SH3BP2
Full Name
SH3 domain-binding protein 2
Weight
62244 MW
Alternative Names
SH3BP2 pS427, phosphorylated SH3BP2, SH3 domain-binding protein 2, SH3BP2 Src homology 3 Binding Protein 2, 3BP-2, RES4-23, 3BP2 SH3BP2 3BP-2, 3BP2, CRBM, CRPM, RES4-23 SH3 domain binding protein 2 SH3 domain-binding protein 2|Abl-SH3 binding protein 2|TNFAIP3 interacting protein 2|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SH3BP2, check out the SH3BP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SH3BP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-SH3BP2 S427 Antibody (P04008)
Hello CJ!
No publications found for P04008
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-SH3BP2 S427 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-SH3BP2 S427 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Phospho-SH3BP2 S427 Antibody
Question
We are currently using anti-Phospho-SH3BP2 S427 antibody P04008 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-Phospho-SH3BP2 S427 antibody (P04008) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
Is a blocking peptide available for product anti-Phospho-SH3BP2 S427 antibody (P04008)?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We do provide the blocking peptide for product anti-Phospho-SH3BP2 S427 antibody (P04008). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-12
Question
Here is the WB image, lot number and protocol we used for cervix using anti-Phospho-SH3BP2 S427 antibody P04008. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-20