Anti-3BP2/SH3BP2 Antibody Picoband™

Product Name

Anti-3BP2/SH3BP2 Antibody Picoband™

View all SH3BP2 Antibodies

SKU/Catalog Number

A04008-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-3BP2/SH3BP2 Antibody Picoband™ catalog # A04008-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-3BP2/SH3BP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04008-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human 3BP2/SH3BP2 recombinant protein (Position: R256-R561).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04008-1 is reactive to SH3BP2 in Human

Applications

A04008-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

62.244kDa

Background of SH3BP2

SH3BP2 (SH3 domain-binding protein 2) is a protein that comes from a gene located on Chromosome 4. The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of 3BP2/SH3BP2 using anti-3BP2/SH3BP2 antibody (A04008-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-3BP2/SH3BP2 antigen affinity purified polyclonal antibody (Catalog # A04008-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 3BP2/SH3BP2 at approximately 70 kDa. The expected band size for 3BP2/SH3BP2 is at 62,70 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-3BP2/SH3BP2 antibody (A04008-1).
Overlay histogram showing JK cells stained with A04008-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-3BP2/SH3BP2 Antibody (A04008-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SH3BP2 (Source: Uniprot.org, NCBI)

Alternative Names

Abl-SH3 binding protein 2; Cherubism; CRBM; CRPM; FLJ42079; FLJ54978,3BP-2,3BP2; RES4-23; SH3 domain-binding protein 2; SH3-domain binding protein 2; TNFAIP3 interacting protein 2 SH3BP2 3BP-2, 3BP2, CRBM, CRPM, RES4-23 SH3 domain binding protein 2 SH3 domain-binding protein 2|Abl-SH3 binding protein 2|TNFAIP3 interacting protein 2|epididymis secretory sperm binding protein

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In this infographic, you will see the following information for SH3BP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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