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Product Info Summary
| SKU: | A15056-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SHCBP1L Antibody Picoband™
SKU/Catalog Number
A15056-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHCBP1L Antibody Picoband™ catalog # A15056-2. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SHCBP1L Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15056-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SHCBP1L recombinant protein (Position: K171-E506).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15056-2 is reactive to Shcbp1l in Human, Rat
Applications
A15056-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
70.948kDa
Background of Shcbp1l
This gene encodes a Src homology 2 domain-binding protein 1-like protein. The encoded protein interacts with heat shock 70 kDa protein 2 and may be involved in maintaining spindle integrity during meiosis. This gene is located in region of chromoso0me 1 encompassing a prostate cancer susceptibility locus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Shcbp1l (Source: Uniprot.org, NCBI)
Gene Name
Shcbp1l
Full Name
Testicular spindle-associated protein SHCBP1L
Weight
70.948kDa
Alternative Names
C1orf14; GE36; MGC26911; SHC SH2 domain-binding protein 1-like protein; SHC SH2-domain binding protein 1-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Shcbp1l, check out the Shcbp1l Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Shcbp1l: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHCBP1L Antibody Picoband™ (A15056-2)
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No publications found for A15056-2
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