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Product Info Summary
| SKU: | A06510-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Siglec-5/14 Antibody
SKU/Catalog Number
A06510-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Siglec-5/14 Antibody catalog # A06510-1. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Siglec-5/14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06510-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2B4
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human Siglec-5/14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06510-1 is reactive to SIGLEC5 in Human
Applications
A06510-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
60.715kDa
Background of SIGLEC5
Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-300
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Spleen, antibody was diluted at 1:100.
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Western Blot (WB) analysis of L929 cells using Siglec-5/14 Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Spleen, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For SIGLEC5 (Source: Uniprot.org, NCBI)
Gene Name
SIGLEC5
Full Name
Sialic acid-binding Ig-like lectin 5
Weight
60.715kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Siglec-5/Siglec-14 SIGLEC5 CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5 sialic acid binding Ig like lectin 5 sialic acid-binding Ig-like lectin 5|CD33 antigen-like 2|OB-binding protein 2|obesity-binding protein 2|sialic acid-binding immunoglobulin-like lectin 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIGLEC5, check out the SIGLEC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIGLEC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Siglec-5/14 Antibody (A06510-1)
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No publications found for A06510-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Siglec-5/14 Antibody
Question
Would A06510-1 anti-Siglec-5/14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
You can see on the product datasheet, A06510-1 anti-Siglec-5/14 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-05
Question
Here is the WB image, lot number and protocol we used for macrophage using anti-Siglec-5/14 antibody A06510-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-Siglec-5/14 antibody A06510-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
The anti-Siglec-5/14 antibody (A06510-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-13
Question
Does anti-Siglec-5/14 antibody A06510-1 work on goat WB with brain?
A. Miller
Verified customer
Asked: 2017-04-04
Answer
Our lab technicians have not validated anti-Siglec-5/14 antibody A06510-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Siglec-5/14 antibody A06510-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-04
