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Product Info Summary
| SKU: | A05580-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-SIK3 Antibody
SKU/Catalog Number
A05580-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIK3 Antibody (Catalog # A05580-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SIK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SIK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05580-1)
Host
Chicken
Contents
SIK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgY
Immunogen
SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3. The immunogen is located within amino acids 1190 - 1240 of SIK3.
Cross-reactivity
At least three isoforms of SIK3 are known to exist; this antibody will detect two isoforms. SIK3 antibody is predicted to not cross-react with SIK1 and SIK2.
Reactive Species
A05580-1 is reactive to SIK3 in Human, Mouse, Rat
Calculated molecular weight
144.9 kDa
Background of SIK3
Salt-inducible kinase family (SIKs) proteins are thought to have a role in steroidogenesis, adipogenesis or regulation of tumor malignancy. Three members (SIK1, SIK2 and SIK3) in the SIK family have been identified thus far. Their kinase domain sequences are closely homologous to those of AMP-activated protein kinase (AMPK). SIK3 can be phosphorylated by a tumor-suppressor kinase LKB1. It is highly and preferentially expressed in ovarian tumors but not in adenomyosis and leiomyoma and may be a potential diagnostic marker for ovarian cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05580-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SIK3 antibody can be used for detection of SIK3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.
Specific Publications For Anti-SIK3 Antibody (A05580-1)
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