Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband™

NHE1/SLC9A1 antibody

Boster Bio Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband™ catalog # PB9151. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9151
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband™

View all NHE1/SLC9A1 Antibodies

SKU/Catalog Number

PB9151

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband™ catalog # PB9151. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9151)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9151 is reactive to SLC9A1 in Human, Mouse, Rat

Applications

PB9151 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

91 kDa

Calculated molecular weight

90.763kDa

Background of NHE1/SLC9A1

NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For SLC9A1 (Source: Uniprot.org, NCBI)

Gene Name

SLC9A1

Full Name

Sodium/hydrogen exchanger 1

Weight

90.763kDa

Superfamily

monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family

Alternative Names

APNH; APNH1; APNHFLJ42224; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na+/H+, amiloride sensitive); Na-Li countertransporter; NHE1; NHE-1; NHE1Na+/H+ antiporter, amiloride-sensitive; SLC9A1; sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter; solute carrier family 9 (sodium/hydrogen exchanger), member 1; Solute carrier family 9 member 1 SLC9A1 APNH, LIKNS, NHE-1, NHE1, PPP1R143 solute carrier family 9 member A1 sodium/hydrogen exchanger 1|Na(+)/H(+) exchanger 1|Na-Li countertransporter|protein phosphatase 1, regulatory subunit 143|solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive)|solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC9A1, check out the SLC9A1 Infographic

SLC9A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC9A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband™

Question

We are currently using anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody PB9151 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody (PB9151) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-13

Question

I see that the anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody PB9151 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-01

Question

Do you have a BSA free version of anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody PB9151 available?

J. Miller

Verified customer

Asked: 2016-06-13

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody PB9151 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-06-13

Question

Will PB9151 anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

F. Rodriguez

Verified customer

Asked: 2014-05-12

Answer

As indicated on the product datasheet, PB9151 anti-Sodium/Hydrogen Exchanger 1/SLC9A1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-05-12

Order DetailsPrice
PB9151

100μg

$370
PB9151-10ug

10μg sample (liquid)

$99
PB9151-Biotin

100 μg Biotin conjugated

$570
PB9151-Cy3

100 μg Cy3 conjugated

$570
PB9151-Dylight488

100 μg Dylight488 conjugated

$570
PB9151-Dylight550

100 μg Dylight550 conjugated

$570
PB9151-Dylight594

100 μg Dylight594 conjugated

$570
PB9151-FITC

100 μg FITC conjugated

$570
PB9151-HRP

100 μg HRP conjugated

$570
PB9151-APC

100 μg APC conjugated

$670
PB9151-PE

100 μg PE conjugated

$670
PB9151-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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