Anti-SLC9A3R2/Nherf 2 Antibody

NHERF-2 antibody

Boster Bio Anti-SLC9A3R2/Nherf 2 Antibody (Catalog # A05624-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05624-1
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-SLC9A3R2/Nherf 2 Antibody

View all NHERF-2 Antibodies

SKU/Catalog Number

A05624-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-SLC9A3R2/Nherf 2 Antibody (Catalog # A05624-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SLC9A3R2/Nherf 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05624-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SLC9A3R2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05624-1 is reactive to SLC9A3R2 in Human, Mouse, Rat

Applications

A05624-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

37.414kDa

Background of NHERF-2

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.

Poulat F., J. Biol. Chem. 272:7167-7172(1997) [PubMed: 9054412].
Reczek D.,J. Cell Biol. 139:169-179(1997) [PubMed: 9314537].
Yun C.H.C., Proc. Natl. Acad. Sci. U.S.A. 94:3010-3015(1997) [PubMed: 9096337].

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For SLC9A3R2 (Source: Uniprot.org, NCBI)

Gene Name

SLC9A3R2

Full Name

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Weight

37.414kDa

Alternative Names

E3KARP; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3RF2; NHERF2MGC104639; NHERF-2NHE3 kinase A regulatory protein E3KARP; OCTS2; SIP-1SRY-interacting protein 1; sodium/hydrogen exchanger; Sodium-hydrogen exchanger regulatory factor 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; TKA-1SIP1; Tyrosine kinase activator protein 1 SLC9A3R2 E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1 SLC9A3 regulator 2 Na(+)/H(+) exchange regulatory cofactor NHE-RF2|NHE3 kinase A regulatory protein E3KARP|NHE3 regulatory factor 2|SRY-interacting protein 1|sodium/hydrogen exchanger 3 kinase A regulatory protein|solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2|solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2|tyrosine kinase activator protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC9A3R2, check out the SLC9A3R2 Infographic

SLC9A3R2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC9A3R2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-SLC9A3R2/Nherf 2 Antibody

Question

Can you help my question with product A05624-1, anti-SLC9A3R2/Nherf 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05624-1 anti-SLC9A3R2/Nherf 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-21

Question

We are currently using anti-SLC9A3R2/Nherf 2 antibody A05624-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

The anti-SLC9A3R2/Nherf 2 antibody (A05624-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-17

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-SLC9A3R2/Nherf 2 antibody A05624-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-03-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-28

Question

Would anti-SLC9A3R2/Nherf 2 antibody A05624-1 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

According to the expression profile of cervix carcinoma erythroleukemia, SLC9A3R2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-SLC9A3R2/Nherf 2 antibody A05624-1 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2017-09-13

Question

Is this A05624-1 anti-SLC9A3R2/Nherf 2 antibody reactive to the isotypes of SLC9A3R2?

J. Lewis

Verified customer

Asked: 2016-09-06

Answer

The immunogen of A05624-1 anti-SLC9A3R2/Nherf 2 antibody is Synthesized peptide derived from internal of human SLC9A3R2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-09-06

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-SLC9A3R2/Nherf 2 antibody A05624-1. Let me know if you need anything else.

L. Brown

Verified customer

Asked: 2013-07-03

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-07-03

Order DetailsPrice
A05624-1-100uL

100uL

$360

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