Anti-Protein SMG7 SMG7 Antibody

SMG7 antibody

Boster Bio Anti-Protein SMG7 SMG7 Antibody (Catalog # A06078). Tested in WB, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A06078
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, WB

Product Name

Anti-Protein SMG7 SMG7 Antibody

View all SMG7 Antibodies

SKU/Catalog Number

A06078

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Protein SMG7 SMG7 Antibody (Catalog # A06078). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Protein SMG7 SMG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06078)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Synthesized peptide derived from internal of human SMG7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06078 is reactive to SMG7 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

127282 MW

Background of SMG7

Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.

Ohnishi T., Mol. Cell 12:1187-1200(2003).
Nagase T., DNA Res. 3:321-329(1996).
Nakajima D., DNA Res. 9:99-106(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A06078 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For SMG7 (Source: Uniprot.org, NCBI)

Gene Name

SMG7

Full Name

Protein SMG7

Weight

127282 MW

Alternative Names

Protein SMG7;EST1-like protein C;SMG-7 homolog;hSMG-7;SMG7;C1orf16, EST1C, KIAA0250; SMG7 C1orf16, EST1C, SGA56M SMG7 nonsense mediated mRNA decay factor protein SMG7|EST1 telomerase component homolog C|EST1-like protein C|breast cancer-associated SGA-56M|ever shorter telomeres 1C|smg-7 homolog, nonsense mediated mRNA decay factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SMG7, check out the SMG7 Infographic

SMG7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Protein SMG7 SMG7 Antibody

Question

Does A06078 anti-SMG7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

You can see on the product datasheet, A06078 anti-SMG7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-17

Question

We are currently using anti-SMG7 antibody A06078 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

The anti-SMG7 antibody (A06078) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-14

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-SMG7 antibody A06078. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-07

Question

Would anti-SMG7 antibody A06078 work for WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

According to the expression profile of leukemic t-cell, SMG7 is highly expressed in leukemic t-cell. So, it is likely that anti-SMG7 antibody A06078 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2019-05-17

Question

Is this A06078 anti-SMG7 antibody reactive to the isotypes of SMG7?

Verified Customer

Verified customer

Asked: 2017-12-11

Answer

The immunogen of A06078 anti-SMG7 antibody is Synthesized peptide derived from internal of human SMG7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-11

Question

See below the WB image, lot number and protocol we used for leukemic t-cell using anti-SMG7 antibody A06078. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-11-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-03

Question

Is a blocking peptide available for product anti-SMG7 antibody (A06078)?

S. Thomas

Verified customer

Asked: 2017-05-31

Answer

We do provide the blocking peptide for product anti-SMG7 antibody (A06078). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-05-31

Order DetailsPrice
A06078-100uL

100uL

$360

Free Secondary Antibody

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A06078
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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