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Product Info Summary
| SKU: | A14601 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SPANX-C Antibody
SKU/Catalog Number
A14601
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-SPANX-C Antibody (Catalog # A14601). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-SPANX-C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14601)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 25-50 of human SPANX protein.
Reactive Species
A14601 is reactive to SPANXC in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
11.0 kDa
Background of SPANXC
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm. Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14601 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
WB: 1:500 - 1:3,000
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~97kDa.
Validation Images & Assay Conditions
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Western blot using Boster's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells in Lane 1. VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein ~97kDa. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
Specific Publications For Anti-SPANX-C Antibody (A14601)
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4 Customer Q&As for Anti-SPANX-C Antibody
Question
We are currently using anti-SPANX-C antibody A14601 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The anti-SPANX-C antibody (A14601) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-14
Question
Is a blocking peptide available for product anti-SPANX-C antibody (A14601)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do provide the blocking peptide for product anti-SPANX-C antibody (A14601). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-06
Question
I was wanting to use your anti-SPANX-C antibody for IHC for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
R. Brown
Verified customer
Asked: 2017-06-14
Answer
You can see on the product datasheet, A14601 anti-SPANX-C antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-14
Question
Do you have a BSA free version of anti-SPANX-C antibody A14601 available?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SPANX-C antibody A14601 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-31
