Anti-Spectrin beta III (SPTBN2) Monoclonal Antibody

Product Name

Anti-Spectrin beta III (SPTBN2) Monoclonal Antibody

View all Spectrin beta 3 Antibodies

SKU/Catalog Number

M05492

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Spectrin beta III (SPTBN2) Monoclonal Antibody (Catalog # M05492). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Spectrin beta III (SPTBN2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05492)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPTBN2/1584

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M05492 is reactive to SPTBN2 in Human, Mouse, Rat

Applications

M05492 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

271.325kDa

Background of Spectrin beta 3

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Pancreas stained with Anti-Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

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Formalin-fixed, paraffin-embedded Rat Pancreas stained with Anti-Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

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Formalin-fixed, paraffin-embedded Mouse Kidney stained with Anti-Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

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Immunofluorescence analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Anti-Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Western Blot analysis of HeLa cell lysate using Anti-Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

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Western Blot analysis of PANC-1 cell lysate using Anti-Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

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Flow Cytometric analysis of HeLa cells using Anti-Spectrin beta III Monoclonal Antibody (SPTBN2/1584) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For SPTBN2 (Source: Uniprot.org, NCBI)

Alternative Names

beta-III Spectrin; glutamate transporter EAAT4-associated protein 41; GTRAP41; KIAA0302; SCA5; SCAR14; Spectrin beta 3; spectrin beta chain, brain 2; Spectrin beta III; spectrin, beta, non-erythrocytic 2; Spectrin, non-erythroid beta chain 2; spinocerebellar ataxia 5; SPTBN2 SPTBN2 GTRAP41, SCA5, SCAR14 spectrin beta, non-erythrocytic 2 spectrin beta chain, non-erythrocytic 2|beta-III spectrin|glutamate transporter EAAT4-associated protein 41|spectrin beta III sigma 2|spectrin beta chain, brain 2|spectrin, non-erythroid beta chain 2|spinocerebellar ataxia 5 protein

For more info on SPTBN2, check out the SPTBN2 Infographic

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In this infographic, you will see the following information for SPTBN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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