Product Info Summary
SKU: | A00604-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TACE ADAM17 Antibody
View all TACE/ADAM17 Antibodies
SKU/Catalog Number
A00604-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TACE ADAM17 Antibody (Catalog # A00604-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
TACE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TACE ADAM17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00604-1)
Host
Rabbit
Contents
TACE Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TACE antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. The immunogen is located within the last 50 amino acids of TACE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.
Reactive Species
A00604-1 is reactive to ADAM17 in Human, Rat
Applications
A00604-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
93.021kDa
Background of TACE/ADAM17
Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25-2 μg/mL; ICC: 10 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples; and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation of TACE in Human Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: TACE (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lanes: HeLa (A,D), Jurkat (B, E), Raji (C,F) in the absence (A-C) or presence (E-F) of blocking peptide.
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KO Validation in HeLa Cells
Loading: 10 μg of HeLa WT cell lysates or TACE KO cell lysates. Antibodies: TACE A00604-1 (0.25 μg/mL) and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: TACE A00604-1 (0.5 μg/mL), TACE (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of TACE in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with A00604-1 at 10 μg/mL
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Immunocytochemistry Validation of TACE in HeLa Cells
Immunohistochemical analysis of HeLa cells using anti-TACE antibody (A00604-1) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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KD Validation of TACE in Monkey COS Cells. (Wang et al., 2006)
COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody (A00604-1). TACE expression levels were markedly reduced in TACE knockdown cell lysate.
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KD Validation of TACE in MDA-MB-435 Cells.
ADAM-17 protein expression, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies (A00604-1).
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Overexpression Validation of TACE in MCF-7 Cells. (McGowan et al., 2007)
ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 (A00604-1) antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells.
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Immunofluorescence Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)
Double immunostaining of control and glutamate-exposed rat cortical cultures. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity.(C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate.
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Immunofluorescence Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)
Double immunostaining of control and glutamate-exposed rat cortical cultures. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity.(C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate.
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Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005)
Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.
Protein Target Info & Infographic
Gene/Protein Information For ADAM17 (Source: Uniprot.org, NCBI)
Gene Name
ADAM17
Full Name
Disintegrin and metalloproteinase domain-containing protein 17
Weight
93.021kDa
Alternative Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme ADAM17 ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE ADAM metallopeptidase domain 17 disintegrin and metalloproteinase domain-containing protein 17|ADAM metallopeptidase domain 18|TNF-alpha convertase|TNF-alpha convertase enzyme|TNF-alpha converting enzyme|a disintegrin and metalloproteinase 17|cartilage snake venom-like protease|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAM17, check out the ADAM17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAM17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TACE ADAM17 Antibody (A00604-1)
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