Product Info Summary
SKU: | A00095 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TCF3/E2A Antibody
SKU/Catalog Number
A00095
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TCF3/E2A Antibody (Catalog # A00095). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCF3/E2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00095)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human TCF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00095 is reactive to TCF3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
67600 MW
Background of TCF-3/E2A
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
Kamps M.P., Cell 60:547-555(1990).
Nourse J., Cell 60:535-545(1990).
Grimwood J., Nature 428:529-535(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00095 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 293 cells, using TCF3 antibody A00095.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue using TCF3 antibody A00095.
Protein Target Info & Infographic
Gene/Protein Information For TCF3 (Source: Uniprot.org, NCBI)
Gene Name
TCF3
Full Name
Transcription factor E2-alpha
Weight
67600 MW
Alternative Names
Transcription factor E2-alpha;Class B basic helix-loop-helix protein 21;bHLHb21;Immunoglobulin enhancer-binding factor E12/E47;Immunoglobulin transcription factor 1;Kappa-E2-binding factor;Transcription factor 3;TCF-3;Transcription factor ITF-1;TCF3;BHLHB21, E2A, ITF1; TCF3 AGM8, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75 transcription factor 3 transcription factor E2-alpha|NOL1-TCF3 fusion|VDR interacting repressor|class B basic helix-loop-helix protein 21|helix-loop-helix protein HE47|immunoglobulin transcription factor 1|kappa-E2-binding factor|negative vitamin D response element-binding protein|transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)|transcription factor 3 variant 3|transcription factor ITF-1|vitamin D receptor-interacting repressor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCF3, check out the TCF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCF3/E2A Antibody (A00095)
Hello CJ!
No publications found for A00095
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TCF3/E2A Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TCF3/E2A Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-TCF3/E2A Antibody
Question
We are currently using anti-TCF3/E2A antibody A00095 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The anti-TCF3/E2A antibody (A00095) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-18
Question
Is a blocking peptide available for product anti-TCF3/E2A antibody (A00095)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We do provide the blocking peptide for product anti-TCF3/E2A antibody (A00095). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-12
Question
Is there a BSA free version of anti-TCF3/E2A antibody A00095 available?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TCF3/E2A antibody A00095 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-13
Question
Please see the WB image, lot number and protocol we used for testis using anti-TCF3/E2A antibody A00095. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-30
Question
Does anti-TCF3/E2A antibody A00095 work on horse WB with lymphoma?
A. Patel
Verified customer
Asked: 2015-11-27
Answer
Our lab technicians have not validated anti-TCF3/E2A antibody A00095 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TCF3/E2A antibody A00095 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lymphoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-27
Question
My question regarding product A00095, anti-TCF3/E2A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Carter
Verified customer
Asked: 2014-01-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00095 anti-TCF3/E2A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-01-17
Question
Would anti-TCF3/E2A antibody A00095 work for IHC with testis?
L. Mangal
Verified customer
Asked: 2013-09-05
Answer
According to the expression profile of testis, TCF3 is highly expressed in testis. So, it is likely that anti-TCF3/E2A antibody A00095 will work for IHC with testis.
Boster Scientific Support
Answered: 2013-09-05