Anti-TCF3/E2A Antibody

Product Name

Anti-TCF3/E2A Antibody

View all TCF-3/E2A Antibodies

SKU/Catalog Number

A00095

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TCF3/E2A Antibody (Catalog # A00095). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TCF3/E2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00095)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human TCF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00095 is reactive to TCF3 in Human, Mouse, Rat

Applications

A00095 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

67.6kDa

Background of TCF-3/E2A

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.

Kamps M.P., Cell 60:547-555(1990).
Nourse J., Cell 60:535-545(1990).
Grimwood J., Nature 428:529-535(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from 293 cells, using TCF3 antibody A00095.

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Immunohistochemistry analysis of paraffin-embedded human brain tissue using TCF3 antibody A00095.

Gene/Protein Information For TCF3 (Source: Uniprot.org, NCBI)

Alternative Names

bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor TCF3 AGM8, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75 transcription factor 3 transcription factor E2-alpha|NOL1-TCF3 fusion|VDR interacting repressor|class B basic helix-loop-helix protein 21|helix-loop-helix protein HE47|immunoglobulin transcription factor 1|kappa-E2-binding factor|negative vitamin D response element-binding protein|transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)|transcription factor 3 variant 3|transcription factor ITF-1|vitamin D receptor-interacting repressor

For more info on TCF3, check out the TCF3 Infographic

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In this infographic, you will see the following information for TCF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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