Product Info Summary
| SKU: | A01052-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TFPI Antibody
SKU/Catalog Number
A01052-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TFPI Antibody (Catalog # A01052-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TFPI antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TFPI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01052-1)
Host
Rabbit
Contents
TFPI Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TFPI antibody was raised against a 13 amino acid peptide near the amino terminus of human TFPI. The immunogen is located within amino acids 70 - 120 of TFPI.
Cross-reactivity
TFPI antibody is human, mouse and rat reactive. At least two isoforms of TFPI are known to exist; this antibody will detect both isoforms.
Reactive Species
A01052-1 is reactive to TFPI in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
35.0 kDa
Background of TFPI
Tissue factor pathway inhibitor (TFPI), also known as lipoprotein-associated coagulation inhibitor, is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation (1). TFPI is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01052-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TFPI antibody can be used for detection of TFPI by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TFPI in rat small intestine tissue lysate with TFPI antibody at 1 μg/mL.
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Immunohistochemistry of TFPI in rat small intestine tissue with TFPI antibody at 5 μg/mL.
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Immunofluorescence of TFPI in rat small intestine tissue with TFPI antibody at 20 μg/mL.
Specific Publications For Anti-TFPI Antibody (A01052-1)
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