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Product Info Summary
| SKU: | A01096-4 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Thymic stromal lymphopoietin TSLP Antibody
SKU/Catalog Number
A01096-4
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Thymic stromal lymphopoietin TSLP Antibody (Catalog # A01096-4). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Mouse, Rat.
Storage & Handling
TSLP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Thymic stromal lymphopoietin TSLP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01096-4)
Host
Rabbit
Contents
TSLP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TSLP antibody was raised against a peptide corresponding to 20 amino acids near the center of mouse TSLP. The immunogen is located within the amino acids 70-120 of TSLP.
Reactive Species
A01096-4 is reactive to TSLP in Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
18.1 kDa
Background of TSLP
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01096-4 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 - 2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Mouse A-20 Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TSLP A01096-4 (A: 0.5 μg/mL, B: 1 μg/mL, C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of TSLP in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with A01096-4 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Immunohistochemistry Validation of TSLP in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-TSLP antibody (A01096-4) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Induction Validation of TSLP in Nasal Mucosa of Allergic Rhinitis (AR) mice (Wang et al., 2012)
Western Blot images showing the
TSLP protein detected by anti-mouse TSLP antibodies in normal mice (Control) or AR mice. TSLP protein expression levels were higher in AR mice and AR-GFP mice.
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Induction Validation of TSLP in Rat VSMCs (Zhao et al., 2012)
Immunohistochemical images showing the TSLP protein detected by anti-TSLP antibodies in Rat VSMCs without (Control) or with treatment with TNF-alpha, Ang II, Ang II+PDTC , Ang II +valsartan. TNF-α treatment is used as a positive control. The production of TSLP was increased after Ang II treatment.
Specific Publications For Anti-Thymic stromal lymphopoietin TSLP Antibody (A01096-4)
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