Anti-Thymidylate Synthase Antibody

Product Name

Anti-Thymidylate Synthase Antibody

View all Thymidylate Synthase Antibodies

SKU/Catalog Number

A04320-2

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Thymidylate Synthase Antibody (Catalog # A04320-2). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Thymidylate Synthase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04320-2)

Host

Sheep

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04320-2 is reactive to TYMS in Human

Applications

A04320-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

35.716kDa

Background of Thymidylate Synthase

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: User optimized
IF Microscopy: User optimized
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract.Each lane is estimated to contain 4 ug of protein.Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody.Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.

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Figure 2. Western blot analysis of TYMS using anti-TYMS antibody (A04320-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYMS antigen affinity purified polyclonal antibody (Catalog # A04320-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Sheep IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for TYMS.

Gene/Protein Information For TYMS (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1.45; HsT422; MGC88736; thymidylate synthase; thymidylate synthetase; TMSTsase; TSase; TSHST422 TYMS HST422, TMS, TS thymidylate synthetase thymidylate synthase|TSase

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