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Product Info Summary
| SKU: | A04320-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-TYSY Antibody(C-term)
SKU/Catalog Number
A04320-3
Size
80 µl
Description
Boster Bio Anti-TYSY Antibody(C-term) (Catalog # A04320-3). Tested in Flow Cytometry, IF, IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TYSY Antibody(C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04320-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human TYSY.
Reactive Species
A04320-3 is reactive to TYMS in Human
Calculated molecular weight
35.7 kDa
Background of TYMS
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04320-3 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:50
WB: 1:2000
IHC-P: 1:500
FC: 1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of TYSY Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Confocal immunofluorescent analysis of TYSY Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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TYSY Antibody (C-term) western blot analysis in Hela,Jurkat and HL-60 cell line lysates (35ug/lane).This demonstrates the TYSY antibody detected the TYSY protein (arrow).
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TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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TYSY Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-TYSY Antibody(C-term) (A04320-3)
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