Click image to see more details
Product Info Summary
| SKU: | A02592 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Transcription factor 12 TCF12 Antibody
SKU/Catalog Number
A02592
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription factor 12 TCF12 Antibody (Catalog # A02592). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TCF12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Transcription factor 12 TCF12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02592)
Host
Rabbit
Contents
TCF12 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TCF12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCF12. The immunogen is located within amino acids 50 - 100 of TCF12.
Reactive Species
A02592 is reactive to TCF12 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
73.0 kDa
Background of TCF12
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02592 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 μg/mL.
Click image to see more details
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 μg/mL.
Specific Publications For Anti-Transcription factor 12 TCF12 Antibody (A02592)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Transcription factor 12 TCF12 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Transcription factor 12 TCF12 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
