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Product Info Summary
| SKU: | A03258 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TRIM24 Antibody
View all TIF1 alpha Antibodies
SKU/Catalog Number
A03258
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM24 Antibody (Catalog # A03258). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRIM24 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TRIM24 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03258)
Host
Rabbit
Contents
TRIM24 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
TRIM24 antibody was raised against a 19 amino acid peptide near the center of human TRIM24. The immunogen is located within amino acids 530 - 580 of TRIM24.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of TRIM24 are known to exist.
Reactive Species
A03258 is reactive to TRIM24 in Human, Mouse, Rat
Applications
A03258 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
116.831kDa
Background of TIF1 alpha
TRIM24 (TIF1), a member of the tripartite motif (TRIM) family, plays a role in the regulation of cell proliferation and apoptosis. TRIM24 localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. TIF1 mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRIM24 antibody can be used for detection of TRIM24 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRIM24 in A431 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of TRIM24 in EL4 cells with TRIM24 antibody at 2.5 μg/ml.
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Immunofluorescence of TRIM24 in EL4 cells with TRIM24 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For TRIM24 (Source: Uniprot.org, NCBI)
Gene Name
TRIM24
Full Name
Transcription intermediary factor 1-alpha
Weight
116.831kDa
Alternative Names
E3 ubiquitin-protein ligase TRIM24; EC 6.3.2; EC 6.3.2.-; hTIF1; PTC6; RING finger protein 82; RNF82Tif1a; TIF1-alpha; TIF1ATIF1ALPHA; TIF1TF1A; transcription intermediary factor 1-alpha; transcriptional intermediary factor 1; tripartite motif containing 24; tripartite motif-containing 24; Tripartite motif-containing protein 24 TRIM24 PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1 tripartite motif containing 24 transcription intermediary factor 1-alpha|E3 ubiquitin-protein ligase TRIM24|RING finger protein 82|RING-type E3 ubiquitin transferase TIF1-alpha|TIF1-alpha|transcriptional intermediary factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM24, check out the TRIM24 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM24: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM24 Antibody (A03258)
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Customer Reviews
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