Anti-TRPM5 Antibody Picoband™

Product Name

Anti-TRPM5 Antibody Picoband™

View all TRPM5 Antibodies

SKU/Catalog Number

PB9903

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRPM5 Antibody Picoband™ catalog # PB9903. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRPM5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9903)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRPM5 recombinant protein (Position: S1021-Q1108). Human TRPM5 shares 87.5% amino acid (aa) sequence identity with mouse TRPM5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9903 is reactive to TRPM5 in Human, Mouse, Rat

Applications

PB9903 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

131 kDa

Calculated molecular weight

131.451kDa

Background of TRPM5

Transient receptor potential cation channel subfamily M member 5 (TRPM5) is a protein that in humans is encoded by the TRPM5 gene. This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca (2+) ions. It is activated by lower concentrations of intracellular Ca (2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SMMC whole cell lysates,
Lane 2: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM5 antigen affinity purified polyclonal antibody (Catalog # PB9903) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM5 at approximately 131 kDa. The expected band size for TRPM5 is at 131 kDa.

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Figure 2. IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
TRPM5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
TRPM5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
TRPM5 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For TRPM5 (Source: Uniprot.org, NCBI)

Alternative Names

Long transient receptor potential channel 5; LTrpC5; LTrpC-5; LTRPC5transient receptor potential cation channel subfamily M member 5; MLSN1- and TRP-related gene 1 protein; MTR1MLSN1 and TRP-related; transient receptor potential cation channel, subfamily M, member 5 TRPM5 LTRPC5, MTR1 transient receptor potential cation channel subfamily M member 5 transient receptor potential cation channel subfamily M member 5|MLSN1 and TRP-related|MLSN1- and TRP-related gene 1 protein|long transient receptor potential channel 5

For more info on TRPM5, check out the TRPM5 Infographic

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In this infographic, you will see the following information for TRPM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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