Anti-U1A SNRPA Antibody

Product Name

Anti-U1A SNRPA Antibody

View all U1A Antibodies

SKU/Catalog Number

A08780-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-U1A SNRPA Antibody catalog # A08780-1. Tested in WB,ICC,IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-U1A SNRPA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08780-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human NEDL2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08780-1 is reactive to SNRPA in Human, Mouse, Rat

Applications

A08780-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

31.28kDa

Background of U1A

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner By similarity. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Boulon S., Mol. Cell 16:777-787(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
IP: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of SNRPA using Anti-U1A SNRPA Antibody (A08780-1).

ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-U1A SNRPA Antibody A08780-1

Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: K562
Lane 3: A431
Lane 4: Mouse kidney
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For SNRPA (Source: Uniprot.org, NCBI)

Alternative Names

Mud1; small nuclear ribonucleoprotein polypeptide A; U1 snRNP A; U1 snRNP-specific protein A; U1-AU1 small nuclear ribonucleoprotein A; U1AU1 small nuclear RNP-specific A SNRPA Mud1, U1-A, U1A small nuclear ribonucleoprotein polypeptide A U1 small nuclear ribonucleoprotein A|U1 small nuclear RNP-specific A|U1 snRNP A|U1 snRNP-specific protein A

For more info on SNRPA, check out the SNRPA Infographic

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In this infographic, you will see the following information for SNRPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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