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Product Info Summary
| SKU: | M01018-5 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
| Host: | Chicken |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody
View all UCH-L1/PGP9.5 Antibodies
SKU/Catalog Number
M01018-5
Size
100ul
Description
Boster Bio Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody catalog # M01018-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01018-5)
Host
Chicken
Contents
Purified IgY preparation with 5mM NaN3 at 20-30 mg/mL
Clonality
Polyclonal
Immunogen
Recombinant full length human UCHL1 purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01018-5 is reactive to UCHL1 in Bovine, Equine, Human, Mouse, Pig, Rat
Applications
M01018-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
24.824kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10,000. IF/ICC and IHC: 1:1,000.
Validation Images & Assay Conditions
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Western blot analysis
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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken
Protein Target Info & Infographic
Gene/Protein Information For UCHL1 (Source: Uniprot.org, NCBI)
Gene Name
UCHL1
Full Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Weight
24.824kDa
Superfamily
peptidase C12 family
Alternative Names
EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1 UCHL1 HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 ubiquitin C-terminal hydrolase L1 ubiquitin carboxyl-terminal hydrolase isozyme L1|epididymis luminal protein 117|epididymis secretory protein Li 53|neuron cytoplasmic protein 9.5|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|ubiquitin thioesterase L1|ubiquitin thiolesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UCHL1, check out the UCHL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UCHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody (M01018-5)
Hello CJ!
M01018-5 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) Antibody
Question
We have observed staining in rat brain. Are there any suggestions? Is anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From literature brain does express UCHL1. From Uniprot.org, UCHL1 is expressed in anterior cingulate cortex, lung muscle, brain, cajal-retzius cell fetal brain cortex, among other tissues. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-10
Question
We have tried in the past anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody for WB on cajal-retzius cell fetal brain cortex in the past. I am using equine, and We are going to use the antibody for IHC next. Our lab want to know about examining cajal-retzius cell fetal brain cortex as well as anterior cingulate cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
I took a look at the website and datasheets of our anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody and it appears that M01018-5 has been validated on equine in both WB and IHC. Thus M01018-5 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in equine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-20
Question
I see that the anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-02
Question
Do you have a BSA free version of anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 available?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
Will anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 work for WB with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
According to the expression profile of anterior cingulate cortex, UCHL1 is highly expressed in anterior cingulate cortex. So, it is likely that anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 will work for WB with anterior cingulate cortex.
Boster Scientific Support
Answered: 2019-05-28
Question
I am interested in to test anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Johnson
Verified customer
Asked: 2019-02-13
Answer
The products we sell, including anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-13
Question
Is this M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody reactive to the isotypes of UCHL1?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The immunogen of M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody is Recombinant full length human UCHL1 purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-08
Question
We were satisfied with the WB result of your anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody. However we have been able to see positive staining in lung muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is UCHL1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Based on literature, lung muscle does express UCHL1. Generally UCHL1 expresses in cytoplasm. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-23
Question
Does M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
You can see on the product datasheet, M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-25
Question
Please see the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5. Please let me know if you require anything else.
K. Edwards
Verified customer
Asked: 2018-07-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-09
Question
I was wanting to use your anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody for WB for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse anterior cingulate cortex identification?
G. Wu
Verified customer
Asked: 2018-02-14
Answer
As indicated on the product datasheet, M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody has been tested for IHC, WB on bovine, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-14
Question
We are currently using anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody M01018-5 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it likely that the antibody can work on pig tissues as well?
C. Yang
Verified customer
Asked: 2015-12-08
Answer
The anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody (M01018-5) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-08
Question
Is a blocking peptide available for product anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody (M01018-5)?
G. Wu
Verified customer
Asked: 2014-12-08
Answer
We do provide the blocking peptide for product anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody (M01018-5). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-08
Question
Can you help my question with product M01018-5, anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Banerjee
Verified customer
Asked: 2013-02-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01018-5 anti-Ubiquitin C-Terminal Hydrolase 1 (UCHL1) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-05
