Anti-Ubiquitin D UBD Antibody

Product Name

Anti-Ubiquitin D UBD Antibody

View all FAT10 Antibodies

SKU/Catalog Number

A01970-3

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Ubiquitin D UBD Antibody catalog # A01970-3. Tested in WB,ICC,IHC applications. This antibody reacts with Human,Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ubiquitin D UBD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01970-3)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Syntaxin 1 around the phosphorylation site of S14.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01970-3 is reactive to UBD in Human, Mouse

Applications

A01970-3 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

18.473kDa

Background of FAT10

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of UBD using Anti-Ubiquitin D UBD Antibody (A01970-3).

ICC staining Ubiquitin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Ubiquitin D UBD Antibody A01970-3

Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1

Gene/Protein Information For UBD (Source: Uniprot.org, NCBI)

Alternative Names

Diubiquitin; FAT10; FAT10diubiquitin; GABBR1; UBD; UBD-3; ubiquitin D; Ubiquitin-like protein FAT10 UBD FAT10, GABBR1-3, UBD ubiquitin D ubiquitin D|diubiquitin|ubiquitin-like protein FAT10

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In this infographic, you will see the following information for UBD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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