Product Info Summary
SKU: | M08608 |
---|---|
Size: | 50μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-UNC45A Antibody (Monoclonal, AbS1)
SKU/Catalog Number
M08608
Size
50μg/vial
Form
Liquid
Description
Boster Bio Anti-UNC45A Antibody (Monoclonal, AbS1) catalog # M08608. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UNC45A Antibody (Monoclonal, AbS1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08608)
Host
Mouse
Contents
Each vial contains 50% glycerol and 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
AbS1
Isotype
IgG1
Immunogen
Recombinant human UNC45.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M08608 is reactive to UNC45A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
103077 MW
Background of UNC45A
Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08608 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1, 000, colorimetric, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of UNC45A expression in MW marker (lane 1), HELA whole cell lysates (lane 2), 3T3 whole cell lysates (lane 3) and PC-12 whole cell lysates (lane 4). UNC45A at 98KD was detected using mouse anti-UNC45A Antigen Affinity purified monoclonal antibody (Catalog # M08608) at 1:1000. The blot was developed using colorimetric method.
Protein Target Info & Infographic
Gene/Protein Information For UNC45A (Source: Uniprot.org, NCBI)
Gene Name
UNC45A
Full Name
Protein unc-45 homolog A
Weight
103077 MW
Alternative Names
Protein unc-45 homolog A;Unc-45A;GCUNC-45;Smooth muscle cell-associated protein 1;SMAP-1;UNC45A;SMAP1; UNC45A GC-UNC45, GCUNC-45, GCUNC45, IRO039700, SMAP-1, SMAP1, UNC-45A unc-45 myosin chaperone A protein unc-45 homolog A|general cell UNC45|smooth muscle cell associated protein-1|unc-45 homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UNC45A, check out the UNC45A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UNC45A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UNC45A Antibody (Monoclonal, AbS1) (M08608)
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No publications found for M08608
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Customer Q&As
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5 Customer Q&As for Anti-UNC45A Antibody (Monoclonal, AbS1)
Question
Will anti-UNC45A antibody (Monoclonal, AbS1) M08608 work on zebrafish WB with lung uterus?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Our lab technicians have not tested anti-UNC45A antibody (Monoclonal, AbS1) M08608 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-UNC45A antibody (Monoclonal, AbS1) M08608 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lung uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
We are currently using anti-UNC45A antibody (Monoclonal, AbS1) M08608 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The anti-UNC45A antibody (Monoclonal, AbS1) (M08608) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-UNC45A antibody (Monoclonal, AbS1) M08608. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-22
Question
Is this M08608 anti-UNC45A antibody (Monoclonal, AbS1) reactive to the isotypes of UNC45A?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The immunogen of M08608 anti-UNC45A antibody (Monoclonal, AbS1) is Recombinant human UNC45. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-13
Question
I see that the anti-UNC45A antibody (Monoclonal, AbS1) M08608 works with WB, what is the protocol used to produce the result images on the product page?
M. Mangal
Verified customer
Asked: 2017-05-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-01