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Product Info Summary
| SKU: | M04720 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-VAC14 Antibody (N-Term)
SKU/Catalog Number
M04720
Size
50 µl
Description
Boster Bio Anti-VAC14 Antibody (N-Term) (Catalog # M04720). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-VAC14 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04720)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This VAC14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 125-159 amino acids from human VAC14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04720 is reactive to VAC14 in Human
Applications
M04720 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
87.973kDa
Background of VAC14
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-VAC14 Antibody (N-Term) at 1:2000 dilution + human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 88 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M04720 staining VAC14 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For VAC14 (Source: Uniprot.org, NCBI)
Gene Name
VAC14
Full Name
Protein VAC14 homolog
Weight
87.973kDa
Superfamily
VAC14 family
Alternative Names
ArPIKfyve; FLJ10305; FLJ36622; MGC149816; protein VAC14 homolog; Tax1 (human T-cell leukemia virus type I) binding protein 1; Tax1 (human T-cell leukemia virus type I) binding protein 2; Tax1-binding protein 2; TAX1BP2FLJ46582; TRXMGC149815; Vac14 homolog (S. cerevisiae) VAC14 ArPIKfyve, TAX1BP2, TRX VAC14 component of PIKFYVE complex protein VAC14 homolog|Vac14, PIKFYVE complex component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VAC14, check out the VAC14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VAC14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VAC14 Antibody (N-Term) (M04720)
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No publications found for M04720
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