Anti-XPB/ERCC3 Antibody Picoband™

Product Name

Anti-XPB/ERCC3 Antibody Picoband™

View all XPB Antibodies

SKU/Catalog Number

A03103-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-XPB/ERCC3 Antibody Picoband™ catalog # A03103-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-XPB/ERCC3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03103-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human XPB recombinant protein (Position: Q665-K715).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03103-1 is reactive to ERCC3 in Human, Mouse, Rat

Applications

A03103-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

89 kDa

Calculated molecular weight

89.278kDa

Background of XPB

XPB (xeroderma pigmentosum type B) is an ATP-dependent DNA helicase in humans that is a part of the TFIIH transcription factor complex. This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of XPB using anti-XPB antibody (A03103-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPB antigen affinity purified polyclonal antibody (Catalog # A03103-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPB at approximately 89KD. The expected band size for XPB is at 89KD.

Gene/Protein Information For ERCC3 (Source: Uniprot.org, NCBI)

Alternative Names

Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; EC 3.6.1; EC 3.6.4.12; ERCC3; excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing); GTF2H; RAD25; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH; Xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB; XPBC; XPBTFIIH 89 kDa subunit ERCC3 BTF2, GTF2H, RAD25, TFIIH, TTD2, XPB ERCC excision repair 3, TFIIH core complex helicase subunit general transcription and DNA repair factor IIH helicase subunit XPB|BTF2 p89|DNA excision repair protein ERCC-3|DNA repair helicase|DNA repair protein complementing XP-B cells|TFIIH 89 kDa subunit|TFIIH basal transcription factor complex 89 kDa subunit|TFIIH basal transcription factor complex helicase XPB subunit|TFIIH p89|TFIIH subunit XPB|basic transcription factor 2 89 kDa subunit|excision repair cross-complementation group 3|excision repair cross-complementing rodent repair deficiency, complementation group 3|xeroderma pigmentosum group B-complementing protein|xeroderma pigmentosum, complementation group B

For more info on ERCC3, check out the ERCC3 Infographic

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In this infographic, you will see the following information for ERCC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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