Product Info Summary
SKU: | A00833 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-YY1 Antibody
SKU/Catalog Number
A00833
Size
100ul
Form
Liquid
Description
Boster Bio Anti-YY1 Antibody catalog # A00833. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-YY1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00833)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 20-70 of Human eEF2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00833 is reactive to YY1 in Human, Mouse, Rat
Applications
A00833 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.713kDa
Background of YY1
Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of YY1 using Anti-YY1 Antibody (A00833).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YY1 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-YY1 Antibody A00833
Western blot analysis of YY1 on different lysates using anti-YY1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For YY1 (Source: Uniprot.org, NCBI)
Gene Name
YY1
Full Name
Transcriptional repressor protein YY1
Weight
44.713kDa
Superfamily
YY transcription factor family
Alternative Names
Delta transcription factor; DELTA; INO80SYY-1; NF-E1; NF-E1INO80 complex subunit S; transcriptional repressor protein YY1; UCRBP; Yin and Yang 1 protein; Yin and yang 1; YIN-YANG-1; YY1 transcription factor; YY1 YY1 DELTA, GADEVS, INO80S, NF-E1, UCRBP, YIN-YANG-1 YY1 transcription factor transcriptional repressor protein YY1|INO80 complex subunit S|YY-1|Yin and Yang 1 protein|delta transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YY1, check out the YY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YY1 Antibody (A00833)
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No publications found for A00833
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Customer Reviews
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Customer Q&As
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