Anti-ZAC/Plagl1 Antibody Picoband™

Product Name

Anti-ZAC/Plagl1 Antibody Picoband™

View all PLAGL1 Antibodies

SKU/Catalog Number

A03220-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ZAC/Plagl1 Antibody Picoband™ catalog # A03220-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ZAC/Plagl1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03220-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse ZAC/Plagl1 recombinant protein (Position: M1-Q527).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03220-2 is reactive to PLAGL1 in Mouse

Applications

A03220-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

50.819kDa

Background of PLAGL1

Zinc finger protein PLAGL1 is a protein that in humans is encoded by the PLAGL1 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within regulation of gene expression and skeletal muscle cell differentiation. Predicted to be located in Golgi apparatus and nuclear body. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; heart; and sensory organ. Orthologous to human PLAGL1 (PLAG1 like zinc finger 1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Mouse
Flow Cytometry, 1-3 µg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ZAC/Plagl1 using anti-ZAC/Plagl1 antibody (A03220-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZAC/Plagl1 antigen affinity purified polyclonal antibody (Catalog # A03220-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZAC/Plagl1 at approximately 51 kDa. The expected band size for ZAC/Plagl1 is at 51 kDa.

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Figure 2. IF analysis of ZAC/Plagl1 using anti-ZAC/Plagl1 antibody (A03220-2).
ZAC/Plagl1 was detected in an immunocytochemical section of RM1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZAC/Plagl1 Antibody (A03220-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-ZAC/Plagl1 antibody (A03220-2).
Overlay histogram showing ANA-1 cells stained with A03220-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZAC/Plagl1 Antibody (A03220-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PLAGL1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp781P1017; LOT-1; LOT1ZACLost on transformation 1; MGC126275; MGC126276; PLAG-like 1; pleiomorphic adenoma gene-like 1; pleiomorphic adenoma gene-like protein 1; Pleiomorphic adenoma-like protein 1; Tumor supressor ZAC; ZAC tumor supressor; ZAC1; zinc finger protein PLAGL1 PLAGL1 LOT1, ZAC, ZAC1 PLAG1 like zinc finger 1 zinc finger protein PLAGL1|PLAG-like 1|lost on transformation 1|pleiomorphic adenoma gene-like 1|tumor suppressor ZAC

For more info on PLAGL1, check out the PLAGL1 Infographic

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In this infographic, you will see the following information for PLAGL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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