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Product Info Summary
| SKU: | A04754 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ChIP, WB |
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Product info
Product Name
Anti-ZBTB33 Antibody
SKU/Catalog Number
A04754
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ZBTB33 Antibody catalog # A04754. Tested in WB,ChIP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZBTB33 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04754)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human Kaiso/Kaiso/ZBTB33(NP_006768.1).
Reactive Species
A04754 is reactive to ZBTB33 in Human, Mouse, Rat
Calculated molecular weight
74.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04754 is guaranteed for ChIP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
ChIP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using Kaiso/Kaiso/ZBTB33 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.
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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso/ZBTB33 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Specific Publications For Anti-ZBTB33 Antibody (A04754)
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