Product Info Summary
SKU: | A13957 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-Zinc finger protein 174 ZNF174 Antibody
SKU/Catalog Number
A13957
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein 174 ZNF174 Antibody (Catalog # A13957). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zinc finger protein 174 ZNF174 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13957)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZNF174.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13957 is reactive to ZNF174 in Human
Applications
A13957 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
46.455kDa
Background of ZNF174
Transcriptional repressor.
Williams A.J., J. Biol. Chem. 270:22143-22152(1995).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Rush J., Nat. Biotechnol. 23:94-101(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, using ZNF174 antibody A13957.
Click image to see more details
Immunofluorescence analysis of HUVEC cells, using ZNF174 antibody A13957.
Protein Target Info & Infographic
Gene/Protein Information For ZNF174 (Source: Uniprot.org, NCBI)
Gene Name
ZNF174
Full Name
Zinc finger protein 174
Weight
46.455kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
AW-1; zinc finger protein 174; ZSCAN8Zinc finger and SCAN domain-containing protein 8 ZNF174 ZSCAN8 zinc finger protein 174 zinc finger protein 174|AW-1|zinc finger and SCAN domain-containing protein 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF174, check out the ZNF174 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF174: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zinc finger protein 174 ZNF174 Antibody (A13957)
Hello CJ!
No publications found for A13957
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-Zinc finger protein 174 ZNF174 Antibody
Question
Please see the WB image, lot number and protocol we used for cervix lung using anti-ZNF174 antibody A13957. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-12
Question
Can you help my question with product A13957, anti-ZNF174 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13957 anti-ZNF174 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-17
Question
We are currently using anti-ZNF174 antibody A13957 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
M. Jones
Verified customer
Asked: 2015-07-27
Answer
The anti-ZNF174 antibody (A13957) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-27