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Product Info Summary
|Description:||Transient overexpression lysate of actin related protein M1 (ARPM1)|
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ARPM1 (ACTRT3) (NM_032487) Human Over-expression Lysate
Transient overexpression lysate of actin related protein M1 (ARPM1)
Storage & Handling
Cite This Product
ARPM1 (ACTRT3) (NM_032487) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084517)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ARPM1 (ACTRT3) Human Over-expression Lysates NM_032487.
Western blot validation of overexpression lysate (LS084517) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ACTRT3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ACTRT3 (Source: Uniprot.org, NCBI)
Actin-related protein T3
actin related protein M1; actin-related protein M1; MGC15664 ACTRT3 ARP-T3, ARPM1 actin related protein T3 actin-related protein T3|actin-related protein M1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on ACTRT3, check out the ACTRT3 Infographic
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In this infographic, you will see the following information for ACTRT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS084517
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