ATP6AP1 (NM_001183) Human Over-expression Lysate

Product Name

ATP6AP1 (NM_001183) Human Over-expression Lysate

See all ATP6AP1 products

SKU/Catalog Number

LS000537

Size

100 µg

Description

Transient overexpression lysate of ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1)

Storage & Handling

Cite This Product

ATP6AP1 (NM_001183) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000537)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for ATP6AP1 (Source: Uniprot.org, NCBI)

Alternative Names

16A; Ac45; ATP6IP1V-ATPase subunit S1; ATP6S1ORF; ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1; ATPase, H+ transporting, lysosomal accessory protein 1; H+ transporting, lysosomal interacting protein 1; H-ATPase subunit; MGC129781; Protein XAP-3; Vacuolar proton pump subunit S1; VATPS1V-ATPase Ac45 subunit; V-type proton ATPase subunit S1; XAP-3; XAP3V-ATPase S1 accessory protein ATP6AP1 16A, ATP6IP1, ATP6S1, Ac45, CF2, VATPS1, XAP-3, XAP3 ATPase H+ transporting accessory protein 1 V-type proton ATPase subunit S1|ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1|ATPase, H+ transporting, lysosomal accessory protein 1|ATPase, H+ transporting, lysosomal interacting protein 1|H-ATPase subunit|V-ATPase Ac45 subunit|V-ATPase S1 accessory protein|V-ATPase subunit S1|epididymis secretory sperm binding protein|protein XAP-3|vacuolar proton pump subunit S1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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