Avidin-Biotin-Peroxidase Complex for ELISA

Avidin is a protein derived from both avians and amphibians that shows considerable affinity for biotin, a co-factor that plays a role in multiple eukaryotic biological processes. Avidin and other biotin-binding proteins, including Streptavidin and NeutrAvidin protein, have the ability to bind up to four biotin molecules.
The bond formation between biotin and Avidin is very rapid, and once formed, is unaffected by extremes of pH, temperature, organic solvents and other denaturing agents. Cited in 7 publication(s).

Product Info Summary

SKU: AR1103
Size: 100 µl

Product NameAvidin-Biotin-Peroxidase Complex for ELISA
SKU/Catalog NumberAR1103
Pack Size100 µl
Form SuppliedConcentrated solution (100X)
Storage InstructionsStore at -20˚C for one year from date of receipt. Avoid repeated freeze-thaw cycles.
Dilution ratio1:100
ApplicationAvidin-Biotin-Peroxidase Complex (ABC-HRP) reacts with HRP substrate to produce a blue color product. The complex can be used for in vitro quantitative and qualitative analysis of enzyme-linked immunoassays.

Protocol

1.Bring the vial to room temperature (18-25°C) prior to use.
2.Centrifuge the vial prior to use.
3.Add 10 µl of Avidin-Biotin-Peroxidase Complex into 990 µl of PBS containing 2% BSA.
4.Add 100 µl of the prepared 1x Avidin-Biotin-Peroxidase Complex into each well. Cover with the plate sealer provided and incubate for 30 minutes at 37°C.
5.Wash the plate after the incubation.
6.Add 90 µl of HRP substrate to each well and incubate in the dark at 37°C.

Validation Images & Assay Conditions

Hello CJ!

AR1103 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of Intrathecal Injection of Prednisolone Acetate on Expression of NR2B Subunit and nNOS in Spinal Cord of Rats after Chronic Compression of Dorsal Root Ganglia

Changes in tumor necrosis factor alpha and myeloper-oxidase in mouse models of local cerebral infarction induced by photochemical method*

Traditional Chinese medicine Bao Gan Ning increase phosphorylation of CREB in liver fibrosis in vivo and in vitro

Quantitative Determination of Butocarboxim in Agricultural Products Based on Biotinylated Monoclonal Antibody

Silicon, an important exposure marker in vivo in silicosis research

Role of N‐methyl‐D‐aspartate Receptor in Hyperoxia‐Induced Lung Injury

Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates
Species: Rat

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