Angiogenin Antibodies

Angiogenin (ANG)

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA.

Cleaves tRNA within anticodon loops to generate tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues.

Gene Information

Human
Gene Name:	ANG
Uniprot:	P03950
Entrez:	283

Family

Belongs to:
pancreatic ribonuclease family

Alternative Names

ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4

Molecular Weight

Mass (kDA):
16.55 kDA

Genomic Context

Human
Location:	14q11.2
Sequence:	14; NC_000014.9 (20684177..20694186)

Tissue Specificity

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

Subcellular Localizations

Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA (PubMed:12051708).

Diseases

• Hypertensive Disease
• Hypertrophy
• Pathologic Vasoconstriction
• Fibrosis
• Kidney Diseases
• Heart Failure
• Inflammation
• Cardiovascular Diseases
• Cardiac Hypertrophy
• Diabetes Mellitus
• Atherosclerosis
• Disease Of Adrenal Medulla
• Tumor Angiogenesis

• Nervousness
• Stenosis
• Infarction
• Myocardial Infarction
• Proteinuria Of Undiagnosed Cause
• Neoplasms
• Pathologic Neovascularization

Interacting Proteins

• ACTN2
• ANXA2
• FST
• PCNA
• PLAUR

• RNH1
• S100A10

Pathways

• Secretion
• Excretion
• Vasoconstriction
• Pathogenesis
• Glomerular Filtration
• Angiogenesis
• Cell Proliferation
• Localization
• Cell Growth
• Transport
• Vasodilation
• Reflex
• Natriuresis

• Aldosterone Secretion
• Aging
• Regulation Of Blood Pressure
• Cell Death
• Cell Cycle
• Diuresis
• Cell Migration

PTMs

• Phosphorylation
• Cleavage
• Oxidation
• Reduction
• Dephosphorylation
• Nitration
• Glycosylation
• Acetylation
• Iodination
• Myristoylation

• Ribosylation
• Hydroxylation
• Ubiquitination
• Methylation
• Phosphorothioation
• Decarboxylation
• Carboxylation
• Geranylgeranylation
• Deglycosylation
• Prenylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ANG

Boster Bio - Best Uses Of The ANG Marker

When optimizing experiments using the Boster Bio, you can find useful tips and tricks to help you maximize your results. You can also consult an optimization guide to help you decide which controls and procedures to use to optimize your experiments. While every researcher will experience some form of error, proper controls can remove many of the common causes of errors. Troubleshooting guides will help you identify and eliminate potential sources of error.

ANG Marker

When writing in Korean, you can use ang as the main focus of your sentence. This is similar to using the definite article the in English. The ang before the word in Korean means 'babae', and it functions like a "the" in English. You can also use the ang before a pseudo-verb in your sentences to make it clearer that it is the focus of the sentence.

IHC protocol

IHC is widely used in various laboratories to visualize the distribution of cellular components inside cells and in the context of tissue. There are several methods of localizing antigens and selecting an appropriate one requires evaluating specimen type and assay sensitivity. Sample preparation is of utmost importance for antigen exhibition. Tissue samples should be prepared with care for optimal antigen exhibition. Below are some useful tips for sample preparation.

Follow the protocol carefully to achieve the highest quality staining. An inadequate staining is both frustrating and unintelligible. The quality of the antibody, the IHC protocol, and the tissue preparation are all critical to the success of the experiment. In addition, poor sample preparation will have a detrimental effect on the results. Fortunately, there is a troubleshooting checklist that can help you avoid these problems.

Tissue samples are available from several sources. An animal model, biopsy, or surgery will provide fresh tissue. For experiments in animals, the tissue sample should be preserved at least two hours after death. The autopsy specimen, however, is more of a postmortem autolysis specimen, and it should be fixed as soon as possible. Otherwise, antigens may denature, and the analysis will be inaccurate. The IHC protocol for the ANG marker has many advantages.

After the tissue section is prepared, it is embedded in individual wells of a 96-well plate containing 50-75 mL of ABS. Care should be taken to ensure that the sections are fully immersed in the ABS, and that the sections are not adhered to the walls. IHC steps are performed in the dark at room temperature on a medium-high orbital shaker. The duration of primary antibody incubation is dependent on the thickness of the section, ranging from 48 to 72 hr.

Application

Boster Bio is a company devoted to developing high-quality antibodies. They offer multiple platforms for antibody testing, and their proprietary technology provides high-sensitivity ELISA kits. Ang II is an important marker in the development of liver fibrosis, as it is found in the heart tissue of mice. The company also offers rewards for first reviewers of its products, including product credits and a royalty-free license.

In addition, the use of this biomarker may have clinical applications. As an Ang II-induced hepatic fibrosis model, this test may be used to identify potential targets for therapies. Further investigation is necessary to validate this new marker for liver fibrosis. The results from these experiments show promising potential for treating this disease. The researchers believe that a blocker of ASK1 might help treat liver fibrosis.

Ang II treatment increased the expression of a-SMA, a marker of activated HSCs. The treatment also increased the mRNA for activated HSC markers. Moreover, Ang II treatment increased the expression of a-SMA and Col I in activated HSC cells. However, the results showed that the treatment of Ang II increased the expression of both a-SMA and Col I and III in the cocultured cells. This study also demonstrated the utility of this biomarker in evaluating cancer stem cell therapies.

Optimization

Boster Bio optimization guides can be a valuable tool to scientists who are trying to optimize their experiments. These guides can answer a wide range of questions and help scientists optimize their experiments. The information found within them is crucial for improving results and informing decisions. Unfortunately, no experiment runs perfectly, and every researcher will have problems at some point. The good news is that many common causes of error can be eliminated by using proper controls. Troubleshooting guides can also help identify potential sources of error, allowing you to correct them before the experiment is conducted.

Troubleshooting

To troubleshoot the ANG Marker, first diagnose the problem and the circumstances surrounding it. Identify the part of the problem that is not reproducing. Then, develop a plan of attack and keep testing it until it's resolved. If the problem is not resolved, adjust, replace, or repair the cause of the problem. If these don't work, try another method. If the previous method fails, you can try the split-half approach.

Documentation is essential for troubleshooting. You can build a checklist from the information gathered by gathering the information. The technical manual of the device is a great source of tips and tricks. Troubleshooting guides can also provide helpful information. You can also ask for assistance from other subject matter experts or conduct a web search for troubleshooting tips. Troubleshooting is the process of diagnosing and solving problems by systematically working through all the possible causes.