Anti-Angiogenin/Ang Antibody Picoband™

Product Name

Anti-Angiogenin/Ang Antibody Picoband™

View all Angiogenin Antibodies

SKU/Catalog Number

A00146-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiogenin/Ang Antibody Picoband™ catalog # A00146-3. Tested in ELISA, WB applications. This antibody reacts with Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Angiogenin/Ang Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00146-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat Angiogenin/Ang recombinant protein (Position: Q25-L145).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00146-3 is reactive to ANG in Rat

Applications

A00146-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

16.55kDa

Background of Angiogenin

Angiogenin (ANG) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene. Predicted to enable several functions, including actin binding activity; heparin binding activity; and protein homodimerization activity. Involved in liver development. Predicted to be located in several cellular components, including growth cone; neuronal cell body; and nucleolus. Predicted to be part of angiogenin-PRI complex. Human ortholog(s) of this gene implicated in diabetic retinopathy; myocardial infarction; neurodegenerative disease (multiple); and prostate cancer. Orthologous to human ANG (angiogenin).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Rat
Direct ELISA, 0.1-0.5 µg/ml, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Angiogenin/Ang using anti-Angiogenin/Ang antibody (A00146-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiogenin/Ang antigen affinity purified polyclonal antibody (Catalog # A00146-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiogenin/Ang at approximately 25 kDa. The expected band size for Angiogenin/Ang is at 17-22 kDa.

Gene/Protein Information For ANG (Source: Uniprot.org, NCBI)

Alternative Names

ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4 ANG ALS9, HEL168, RAA1, RNASE4, RNASE5 angiogenin angiogenin|RNase 5|angiogenin, ribonuclease, RNase A family, 5|epididymis luminal protein 168|ribonuclease 5|ribonuclease A A1|ribonuclease A family member 5

For more info on ANG, check out the ANG Infographic

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In this infographic, you will see the following information for ANG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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