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Product Info Summary
| SKU: | A00146-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Angiogenin/Ang Antibody Picoband®
SKU/Catalog Number
A00146-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiogenin/Ang Antibody Picoband® catalog # A00146-3. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Angiogenin/Ang Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00146-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Angiogenin/Ang recombinant protein (Position: Q25-L145).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00146-3 is reactive to Ang in Rat
Observed Molecular Weight
25 kDa
Background of Ang
Angiogenin (ANG) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene. Predicted to enable several functions, including actin binding activity; heparin binding activity; and protein homodimerization activity. Involved in liver development. Predicted to be located in several cellular components, including growth cone; neuronal cell body; and nucleolus. Predicted to be part of angiogenin-PRI complex. Human ortholog(s) of this gene implicated in diabetic retinopathy; myocardial infarction; neurodegenerative disease (multiple); and prostate cancer. Orthologous to human ANG (angiogenin).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00146-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, Rat
Positive Control
WB: rat liver tissue
Validation Images & Assay Conditions
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Western blot analysis of Angiogenin/Ang using anti-Angiogenin/Ang antibody (A00146-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiogenin/Ang antigen affinity purified polyclonal antibody (Catalog # A00146-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiogenin/Ang at approximately 25 kDa. The expected band size for Angiogenin/Ang is at 17-22 kDa.
Specific Publications For Anti-Angiogenin/Ang Antibody Picoband® (A00146-3)
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