Anti-Angiogenin/ANG Antibody Picoband™

Boster Bio Anti-Angiogenin/ANG Antibody Picoband™ catalog # A00146. Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00146
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Angiogenin/ANG Antibody Picoband™

See all Angiogenin products

SKU/Catalog Number

A00146

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiogenin/ANG Antibody Picoband™ catalog # A00146. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiogenin/ANG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00146)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00146 is reactive to ANG in Human

Applications

A00146 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ANG (Source: Uniprot.Org, NCBI)

Uniprot ID

P03950

Gene ID

283

Gene Name

ANG

Full Name

Angiogenin

Weight

16.55kDa

Superfamily

pancreatic ribonuclease family

Alternative Names

ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4 ANG ALS9, HEL168, RAA1, RNASE4, RNASE5 angiogenin angiogenin|RNase 5|angiogenin, ribonuclease, RNase A family, 5|epididymis luminal protein 168|ribonuclease 5|ribonuclease A A1|ribonuclease A family member 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANG, check out the ANG Infographic

ANG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00146

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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

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15 Customer Q&As for Anti-Angiogenin/ANG Antibody Picoband™

Question

Would A00146 anti-Angiogenin/ANG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Miller

Verified customer

Asked: 2020-04-14

Answer

As indicated on the product datasheet, A00146 anti-Angiogenin/ANG antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-14

Question

My boss were happy with the WB result of your anti-Angiogenin/ANG antibody. However we have seen positive staining in liver cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is ANG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

Based on literature, liver does express ANG. Generally ANG expresses in cytoplasmic vesicle, secretory vesicle lumen. Regarding which tissues have ANG expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-17

Question

My question regarding product A00146, anti-Angiogenin/ANG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00146 anti-Angiogenin/ANG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-31

Question

Is there a BSA free version of anti-Angiogenin/ANG antibody A00146 available?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Angiogenin/ANG antibody A00146 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-16

Question

We need to test anti-Angiogenin/ANG antibody A00146 on human small intestine for research purposes, then I may be interested in using anti-Angiogenin/ANG antibody A00146 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

The products we sell, including anti-Angiogenin/ANG antibody A00146, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-06

Question

Is a blocking peptide available for product anti-Angiogenin/ANG antibody (A00146)?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

We do provide the blocking peptide for product anti-Angiogenin/ANG antibody (A00146). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-21

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine using anti-Angiogenin/ANG antibody A00146. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-26

Question

We are currently using anti-Angiogenin/ANG antibody A00146 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

S. Li

Verified customer

Asked: 2019-02-07

Answer

The anti-Angiogenin/ANG antibody (A00146) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-07

Question

I was wanting to use your anti-Angiogenin/ANG antibody for ELISA for human small intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human small intestine identification?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

You can see on the product datasheet, A00146 anti-Angiogenin/ANG antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-18

Question

We have seen staining in human liver. Are there any suggestions? Is anti-Angiogenin/ANG antibody supposed to stain liver positively?

S. Jha

Verified customer

Asked: 2018-06-26

Answer

Based on literature liver does express ANG. Based on Uniprot.org, ANG is expressed in liver, small intestine, among other tissues. Regarding which tissues have ANG expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-06-26

Question

I see that the anti-Angiogenin/ANG antibody A00146 works with ELISA, what is the protocol used to produce the result images on the product page?

M. Mitchell

Verified customer

Asked: 2018-03-13

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-13

Question

We ordered your anti-Angiogenin/ANG antibody for WB on liver in a previous experiment. I am using human, and We want to use the antibody for ELISA next. you antibody examining liver as well as small intestine in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2018-01-10

Answer

I looked at the website and datasheets of our anti-Angiogenin/ANG antibody and it seems that A00146 has been validated on human in both WB and ELISA. Thus A00146 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-01-10

Question

Does anti-Angiogenin/ANG antibody A00146 work for ELISA with small intestine?

B. Johnson

Verified customer

Asked: 2017-04-06

Answer

According to the expression profile of small intestine, ANG is highly expressed in small intestine. So, it is likely that anti-Angiogenin/ANG antibody A00146 will work for ELISA with small intestine.

Boster Scientific Support

Answered: 2017-04-06

Question

See attached the WB image, lot number and protocol we used for small intestine using anti-Angiogenin/ANG antibody A00146. Please let me know if you require anything else.

K. Krishna

Verified customer

Asked: 2015-08-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-08-25

Question

Is this A00146 anti-Angiogenin/ANG antibody reactive to the isotypes of ANG?

L. Collins

Verified customer

Asked: 2014-11-04

Answer

The immunogen of A00146 anti-Angiogenin/ANG antibody is E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-11-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00146
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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